\n\nResults: Findings of the current study indicated that the size and shape of silver nanoparticles depended on the type of starch. For example for a special type of potato starch the cubic silver nanoparticles Nepicastat mouse were obtained whereas for other types of potato starch and corn starch spherical silver nanoparticles were produced. Nanoparticle sizes were mainly

about 20 nm, but ranging from 2-30 nm in different situations. Due to the biocompatibility of starch synthesized silver nanoparticles, this method can be used for medicinal purposes. Both kinds of nanosilver containing starch sources have excellent antibacterial activity.\n\nConclusions: The current study indicated that silver nanoparticles produced using different starches had strong antibacterial activities on the studied standard and pathogenic bacteria including gram positive and gram negative.”
“Trunk phloem necrosis (TPN), a physiological bark disorder of the rubber tree (Hevea brasiliensis), is a serious CDK inhibitor problem that affects the yield of natural rubber. The resultant bark dryness occurs in up to half of a plantation’s trees in almost every rubber tree plantation region, causing a great annual loss of dry rubber for natural rubber production. Different types of injury and physical damage caused by mechanical activation as well as environmental stresses cause physiological bark disorder in tree. Due to the essential role of miR166, miR393 and miR167

in vascular development and abiotic stress response in diverse plant species, it was interesting to investigate the role of these miRNAs in rubber trees, particularly during

development of a physiological bark disorder. In this study, the expression pattern of miR166, miR393 and miR167; and their target genes, HD-ZIP III; TIR1 and ARF8, respectively; was demonstrated in selleck kinase inhibitor healthy tree and different TPN trees. Their existence and function in vivo was validated using RNA ligase-mediated 5′ rapid amplification of cDNA ends. Taken together, the results suggest a possible dual role of these three miRNAs in maintaining normal bark regeneration in healthy trees, coping with overtapping by affecting the wound healing system leading to abnormal bark regeneration in overtapped-TPN trees, and act as additional forces that enhance the attenuation of vascular development resulting in bark necrosis and cell death in the natural-TPN tree. This is the first study to address the molecular events of miRNAs involved in the physiological bark disorder TPN in rubber tree. Further study will open the possibility to better understanding of physiological and molecular perspectives during TPN development, and lead to improvement of monitoring the exploitation of rubber tree plantations. (C) 2014 Elsevier GmbH. All rights reserved.”
“The discovery of three new species of Enyalioides from the tropical Andes in Ecuador and northern Peru is reported. Enyalioides altotambo sp. n.

Although both pathogens were effectively inactivated by the dry PL treatments, the appearance of the blueberries was see more adversely affected and a maximum temperature of 64.8 degrees C on the blueberry surface was recorded. On the other hand, the visual appearance of blueberries remained unchanged after wet PL treatments and sample heating was significantly reduced. The wet PL treatments were more effective than chlorine washing on inactivating both pathogens. After a 60-s wet PL treatment, the populations of E. coli 0157:H7 inoculated on calyx and skin of blueberries were reduced by 3.0 and bigger than 5.8 log CFU/g, respectively. Salmonella on blueberry calyx and skin was reduced by 3.6 and

bigger than 5.9 log CFU/g, respectively. No viable bacterial cells were recovered from the water used in the wet PL treatments, demonstrating that this setup could prevent the risk of cross-contamination during fresh produce washing. Our results suggest that this new water-assisted PL treatment could be a potential non-chemical

alternative (residue free) to chlorine washing since it is both more effective and environmentally friendly than chlorine washing. (C) 2013 Elsevier Ltd. All rights reserved.”
“Canada’s oil sands reserves found in an area of about 142,000 km(2) in Northern Alberta are estimated Danusertib research buy to be about 170 billion barrels. Extraction procedures generates oil sands process-affected materials (OSPM), a high pH, high salt and low nutrient residue which contains phytotoxic hydrocarbons. Efficient

methods are needed to revegetate impacted landscapes in line with governmental standards. Actinorhizal alders could be used to provide nitrogen in a reclamation program as they are early successional species BMS-777607 purchase that naturally colonize harsh habitats and improve the soil conditions. In order to evaluate the extent to which alders can withstand OSPM-generated stress, the growth and development of both green (Alnus viridis (Chaix) DC. ssp. crispa) and speckled (Alnus incana (L.) Moench ssp. rugosa) alders in varying proportions of OSPM mixed with pristine sand (from 0 to 100%) was assessed both in the presence and the absence of their symbiont, Frankia sp. Alder survival was not impaired by the presence of OSPM, although symbiosis establishment was less efficient when the alders grew in pure OSPM residue. Actinorhizal alders are very tolerant to OSPM: when grown in pure OSPM (100%), their dry biomass was 15 times greater than that of non-nodulated alders. Moreover, symbiotic alders allocated a higher proportion of their biomass to aerial parts, regardless of the OSPM percentage, whereas non-symbiotic alders showed a greater biomass investment in roots. When symbiosis was present, the alders thrived and produced a large amount of biomass that was highly correlated to the total nodule biomass.

01), and increased cell death and cellular caspase activity (P<0.01). Western blotting data revealed that SB203580 sensitises cancer cells to 5-FU due to an increase in Bax expression. These findings suggest that p38 MARK is involved in cancer cell survival, and that the inhibition of p38 MAPK can enhance 5-FU to kill colorectal cancer cells.”
“Background: Mutations in PKHD1 cause autosomal recessive Caroli disease, which is a rare congenital disorder involving cystic dilatation of the intrahepatic bile ducts. However, the mutational spectrum

of PKHD1 and the Selleckchem CHIR98014 phenotype-genotype correlations have not yet been fully established.\n\nMethods: Whole exome sequencing (WES) was performed on one twin sample with Caroli disease from a Chinese family from Shandong province. Routine Sanger sequencing was used to validate the WES and to carry out segregation studies. We also described the PKHD1 mutation associated with the genotype-phenotype

of this twin.\n\nResults: A combination of WES and Sanger sequencing Dibutyryl-cAMP molecular weight revealed the genetic defect to be a novel compound heterozygous genotype in PKHD1, including the missense mutation c.2507 T>C, predicted to cause a valine to alanine substitution at codon 836 (c.2507T>C, p.Val836Ala), and the nonsense mutation c.2341C>T, which is predicted to result in an arginine to stop codon at codon 781 (c.2341C>T, p.Arg781*). This compound heterozygous genotype co-segregates with the Caroli disease-affected pedigree members, but is absent in 200 normal chromosomes.\n\nConclusions: Our findings indicate exome

sequencing can be useful in the diagnosis of Caroli disease patients and associate a compound heterozygous genotype in PKHD1 with Caroli disease, which further increases our understanding of the mutation spectrum of PKHD1 in association with Caroli disease.”
“The present Ruboxistaurin study aims to assess the antimutagenic potential of methanol extract and different fractions (hexane, ethyl acetate and butanol) of chickrassy (Chukrasia tabularis), belonging to family meliaceae by employing histidine point reversion assay. The antimutagenic effect was evaluated against mutagens, 4-Nitro-o-phenylenediamine and sodium azide and promutagen, 2-Aminofluorene of TA98 and TA100 strain of Salmonella typhimurium. The co-incubation and pre-incubation mode of treatments were used to evaluate the bioantimutagenic and desmutagenic effects, respectively. From the results obtained, it was clear that methanol extract and its fractions showed more desmutagenic effect than bioantimutagenic effect. The methanol extract was found to be most active in TA98 while ethyl acetate fraction showed good results in TA100 strain against both promutagen and direct acting mutagen. High performance liquid chromatography (HPLC) analysis of methanol extract was carried out for the identification of chemical constituents and the results revealed that catechin, quercetin and rutin have contributed to its antimutagenic activity.

This is a serious and sometimes fatal infection which is transmitted from animals to humans by entering the digestive tract or mucosal membrane. Within the initial 10-12 day period, the patient may experience no or relatively minor symptoms, then the bacteria enters the blood system and causes contamination of the internal organs, especially the liver.\n\nObjectives: This study prospectively evaluated two serological methods, the microscopic agglutination test (MAT) and indirect immunofluorescence assay (IFA) with the aim of identifying leptospiral agents quickly, in order to prevent and control

leptospirosis diseases.\n\nPatients and Methods: In this study, 90 blood samples and their serums, were collected from patients showing clinical symptoms of leptospirosis, and these were analyzed Cell Cycle inhibitor with MAT and IFA tests, to test for the presence of leptospiral agglutinins. In this study we used five serogroups of Leptospira interrogans, which have been identified as the dominant serogroups in Iran (Pomona, Canicola, Grippotyphosa, Icterohaemorrhagiae and Sejro hardjo).\n\nResults: The results obtained showed that when compared to the IFA, the MAT was a more sensitive test. The IFA method also identified certain specific properties of the serogroups; Pomona, Canicola and Icterohaemorragiae, which are detailed in the article.\n\nConclusions: Serological

testing is the most widely used https://www.selleckchem.com/products/3-methyladenine.html method for diagnosing leptospirosis, and the MAT is considered to be the gold standard test for the diagnosis and detection of leptospiral antibodies in both human and animal sera, for the infecting serovar and serogroup. Published by Kowsar Corp, 2012. cc 3.0.”
“This study compiles 100 years of North Sea demersal Napabucasin landings, focusing on the UK, and relating them to historical events and political, technological and economical drivers that influenced demersal fisheries. In the early twentieth century, aided by technological advances, the UK, and in particular England, had unchallenged dominance in North Sea demersal fisheries. Since then, the two World Wars and other political developments

have had a great impact on British fisheries. Between the 1920s and 1960s, English ports shifted their interests away from the North Sea towards highly profitable distant waters, whereas the Scottish fleet relied less on these fishing grounds. Meanwhile, especially in the 1960s, other European countries expanded their fisheries, undermining Britain’s lead. In the 1970s and 1980s, Scotland benefitted from mainly fishing in the North Sea. Firstly, the assertion of 200 nautical miles Exclusive Economic Zones made the distant waters inaccessible to English fleets at a time when England’s fisheries were highly dependent on them. Secondly, the relatively minor activity in the North Sea by the English compared to the Scottish fleets coincided with the establishment of the Common Fisheries Policy.

Ten placentas were harvested at the time of foal delivery and examined both for gross and histological characteristics. The following factors were determined: the total weight

and volume of the placenta and its components, the surface area of the allantochorion, umbilical cord length and site of insertion, and the diameter Epacadostat clinical trial of the umbilical cord vessels and vascular pattern type. The weight of the placenta was similar to previously reported for ponies, and represented 12% of foal birth weight. Umbilical cord length was similar to that in the horse but longer than in the pony, while cord weight was intermediate between the two. In a histological examination, numerous strong villi were observed at sites corresponding to the non-pregnant and pregnant horn and uterine body. No villi were detected in the area overlying the cervical star. Despite obvious similarities between the donkey and horse placenta, specific morphological features do exist,

and are possibly related to the differences in length of gestation. (c) 2008 Elsevier Inc. All rights reserved.”
“For the treatment of patients with acute coronary syndromes in the catheterization laboratory, a high-dose bolus (HDB) regimen of tirofiban (25 mu g/kg bolus, followed by an infusion of 0.15 mu g/kg/min) leads to a consistent and rapid inhibition of platelet aggregation during the first hour after initiation of therapy. The objective of the present study was to use pharmacokinetic modeling to identify an appropriate dosage of tirofiban that would produce a plasma concentration-time profile in patients with JQ1 molecular weight severe renal impairment (creatinine clearance < 30 ml/min) as similar as possible to that of the HDB regimen in patients with normal renal function. For patients with severe renal impairment, previous recommendations

have been to reduce the dosage by 50%. Pharmacokinetic modeling was performed with the following sets of data: the plasma concentrations of tirofiban from patients with normal renal function who were treated with the HDB EPZ-6438 mouse regimen of tirofiban and the plasma concentrations of tirofiban from patients with severe renal impairment who were treated with a 0.1 mu g/kg/min infusion of tirofiban for 1 h. In conclusion, for patients with severe renal impairment, a 25 mu g/kg bolus, followed by a 0.10 mu g/kg/min maintenance infusion of tirofiban produced a plasma concentration-time profile similar to that observed with the HDB regimen of tirofiban in patients with normal renal function. Coron Artery Dis 23:208-214 (C) 2012 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.”
“OBJECTIVES: The transoral atlantoaxial reduction plate system treats irreducible atlantoaxial dislocation from transoral atlantoaxial reduction plate-I to transoral atlantoaxial reduction plate-III.

Commensal strains were engineered to secrete the insulinotropic proteins GLP-1 and PDX-1. Epithelia stimulated by engineered strains and glucose secreted up to 1 ng ml(-1) of insulin with no significant background secretion.”
“Aspirin-exacerbated respiratory disease (AERD) is a clinical syndrome that is characterized by nasal polyposis, general symptoms of asthma and sensitive response to nonsteroidal anti-inflammatory drugs (NSAIDs). Although the exact function of tripartite motif-containing 26 (TRIM26) still remains unknown, LY3039478 Stem Cells & Wnt inhibitor the gene functions in the immune response. Thus, we hypothesized that TRIM26 polymorphisms may affect aspirin-induced

bronchospasm and explored whether the gene can be a marker for diagnosis of AERD. To investigate our hypothesis that TRIM26 may serve as a genetic marker for diagnosis of AERD), this study focused on demonstrating the associations between single nucleotide polymorphisms (SNPs) of the TRIM26 gene and AERD. We genotyped 18 polymorphisms of TRIM26 in a total of 189 asthmatics and examined their associations with the risk of AERD. We performed logistic analysis for obtaining P-values and regression analysis for demonstrating an association between the phenotype with FEV1 and the TH-302 manufacturer genotype. We observed no associations between polymorphisms in TRIM26 and the risk

of AERD in both logistic and regression analyses. Although our results reveal a lack of association, the suggested functional role of TRIM26 makes it a putative candidate gene for AERD. Thus, replications in other populations using larger samples may provide valuable information 4EGI-1 in vivo for AERD etiology.”
“Immunoglobulin A (IgA) has a critical role in immune defense particularly at the mucosal surfaces, and is equipped to do so by the unique structural attributes of its heavy chain and by its ability to polymerize. Here, we provide an overview of human IgA structure,

describing the distinguishing features of the IgA1 and IgA2 subclasses and mapping the sites of interaction with host receptors important for IgA’s functional repertoire. Remarkably, these same interaction sites are targeted by binding proteins and proteases produced by various pathogens as a means to subvert the protective IgA response. As interest in the prospect of therapeutic IgA-based monoclonal antibodies grows, the emerging understanding of the relationship between IgA structure and function will be invaluable for maximizing the potential of these novel reagents.”
“Recurring and spontaneous seizures in epilepsy result from cell signaling aberrations thought to include synaptic reorganization and various neurotransmitter abnormalities, especially gamma-amino butyric acid (GABA) and glutamate. Cyclooxygenase-2 (COX-2) activity produces oxidative stress and results in the production of prostaglandins that have many injurious effects.

007). Patients with p16 overexpression

had poorer survival than patients with p16 underexpression (59.3% vs 83.8% 5-year survival rate, log-rank p=0.015). In univariate analysis, p16 expression was a significant predictor of survival (RR 2.76, p=0.02).\n\nConclusions Results suggest that p16 expression is an important predictive factor of LN metastasis in cervical cancer patients. Moreover, p16 overexpression is associated with a poor prognosis. Therefore, Apoptosis inhibitor immunohistochemical evaluation of p16 expression is of potential value for treatment planning in cervical carcinomas.”
“Bronchial epithelial cells express xenobiotic-metabolizing enzymes (XMEs) that are involved in the biotransformation of inhaled toxic compounds. The activities of these XMEs in the lung may modulate respiratory toxicity and have been linked to several diseases of the airways. Arylamine N-acetyltransferases (NAT) are conjugating XMEs that play a key role in the biotransformation of aromatic amine pollutants such as the tobacco-smoke carcinogens 4-aminobiphenyl (4-ABP) and beta-naphthylamine (beta-NA).

We show here that functional human NAT1 or its murine counterpart Nat2 are present in different lung epithelial cells i.e. Clara cells, type II alveolar cells and bronchial epithelial cells, thus indicating that inhaled aromatic amines may undergo NAT-dependent biotransformation in lung epithelium. Exposure of these cells to pathophysiologically relevant amounts of oxidants GM6001 mw known to contribute to lung dysfunction, such as H(2)O(2) or peroxynitrite, was found to impair the

NAT1/Nat2-dependent cellular biotransformation QNZ price of aromatic amines. Genetic and non genetic impairment of intracellular NAT enzyme activities has been suggested to compromise the important detoxification pathway of aromatic amine N-acetylation and subsequently to contribute to an exacerbation of untoward effects of these pollutants on health. Our study suggests that oxidative/nitroxidative stress in lung epithelia cells, due to air pollution and/or inflammation, could contribute to local and/or systemic dysfunctions through the alteration of the functions of pulmonary NAT enzymes. (C) 2009 Elsevier Inc. All rights reserved.”
“Vitiligo vulgaris is an autoimmune pigmentary disorder with no universally efficacious therapeutic options. Separate applications of calcipotriene ointment 0.005% and topical corticosteroid ointments have been successful in the repigmentation of vitiligo. We sought to examine the efficacy of a combination calcipotriene 0.005%-betamethasone dipropionate 0.064% ointment in the repigmentation of vitiligo. An institutional review board-approved retrospective chart review was conducted in 13 pediatric and adult patients with vitiligo treated with calcipotriene 0.005%-betamethasone dipropionate 0.064% ointment once daily for at least 2 months.

\n\nMethods: A population based, cross-sectional survey was conducted using multi-stage random cluster sampling. C188-9 nmr 54,279 adults participated in the survey with a response rate of 96%. Doctors interviewed and classified participants as suspected TB cases if they presented with persistent cough, abnormal chest X-ray (CXRAY), or both. Three sputum specimens of all suspected cases were collected and sent for smear microscopy and culture.\n\nResults: Adjusted prevalence rate of bacteriologically confirmed cases was 34 per 100,000 for adults in Shandong in

2010. Compared to the 2000 survey, TB prevalence has declined by 80%. 53% of bacteriologically confirmed cases did not present persistent cough. The yield of bacteriologically confirmed cases was 47% by symptom screening and 95% by CXRAY. Over 50% of TB cases were among over 65′s.\n\nConclusions: The prevalence rate of bacteriologically confirmed cases GSK1210151A concentration was significantly reduced compared with 2000. The survey raised challenges to identify TB cases without clear symptoms.”
“BACKGROUND The prognostic value of a 3 wave and ST-segment morphology after 3 wave in inferolateral leads

in Brugada syndrome (BS) is still unknown.\n\nOBJECTIVE To evaluate the prognostic value of a 3 wave and ST-segment morphology after 3 wave in a large Japanese cohort of BS.\n\nMETHODS A total of 460 consecutive patients with BS (mean age 52 +/- 14 years, 432 men) were enrolled. The presence and location of leads showing a 3 wave, ST-segment morphology after 3 wave, and clinical outcomes were evaluated in patients with documented ventricular fibrillation (VF) (N = 84), those with syncope without documented VF (N = 109), and subjects without symptoms (N = 267).\n\nRESULTS The prevalence of a 3 wave in the inferior and/or lateral leads was 12% (53 cases). The prevalence of a 3 wave among the 3

groups was not different. The incidence of cardiac events (sudden cardiac death or VF) during a mean follow-up period of 50 +/- 32 months was not different in patients with (11%) or without (8%) a 3 wave. Patients with a 3 wave in both inferior and lateral leads or with horizontal ST-segment morphology after 3 wave showed a higher incidence of cardiac events than those without (P = .04 and .02, respectively). Multivariate analysis revealed symptoms, Autophagy Compound Library QRS duration in lead V-2 > 90 ms, and inferolateral 3 wave and/or horizontal ST-segment morphology after 3 wave were important for predicting cardiac events.\n\nCONCLUSION The presence of a 3 wave in multiple leads and horizontal ST-segment morphology after 3 wave may indicate a highly arrhythmogenic substrate in patients with BS.”
“A large, multiyear data set was generated by pooling the sediment elutriate (SET) results collected during previous studies conducted in the Galician Rias (northwest Iberian Peninsula) that met the acceptability criteria in the controls, to ensure optimum quality of data (n = 162).

At the end of

procedure, prevention of VT inducibility was achieved in 25 of 35 patients (71.4%) with previously inducible VT; VT was still inducible in 5 of 8 patients with incomplete LP abolition; and in 5 of 42 patients (16.1%) with complete LP abolition (P < 0.01). After a follow-up of 13.4 +/- 4.0 months, 10 patients (20.0%) had VT recurrences and one of them died after surgical VT ablation; VT recurrence was 9.5% in patients with LPs abolition (4/42 pts) and 75.0% (6/8 pts) in those with incomplete abolition [positive predictive value (PPV): 75%, negative predictive value (NPV): 90.4%, sensibility: 60.0%, and specificity: 95.0%, P < 0.0001); although it was 12.5% (5/40 pts) in patients without Pevonedistat datasheet inducibility VT after the ablation, and 50% (5/10 pts) in those with inducible VT (PPV: 50%, NPV: 87.5%, sensitivity: 50.0%, and specificity: 87.5%, P = 0.008). Conclusions: LP abolition is an effective endpoint of VT ablation and its prognostic value compares favorably to that achieved by programmed electrical stimulation.

(J Cardiovasc Electrophysiol, P005091 price Vol. 23, pp. 621627, June 2012)”
“Smooth muscle contraction is activated primarily by phosphorylation at S19 of the 20-kDa regulatory light chain subunits of myosin II (LC20) catalyzed by Ca2+/calmodulin-dependent myosin light chain kinase. Other kinases, for example, integrin-linked kinase (ILK), Rho-associated kinase (ROCK), and zipper-interacting protein kinase (ZIPK), can phosphorylate T18 in addition to S19, which increases the actin-activated myosin MgATPase activity at subsaturating actin concentrations similar to 3-fold. These phosphorylatable residues and the amino acid sequence surrounding them are highly conserved throughout the animal kingdom; they are also found in an LC20 homolog within the genome of Monosiga brevicollis, the closest living relative of metazoans. LC20 diphosphorylation has been detected in mammalian vascular smooth muscle tissues in response

to specific contractile stimuli and in pathophysiological situations associated with hypercontractility. LC20 diphosphorylation has also been observed frequently in ICG-001 supplier cultured cells where it activates force generation. Kinases such as ILK, ROCK, and ZIPK, therefore, are potential therapeutic targets in the treatment of, for example, cerebral vasospasm following subarachnoid hemorrhage and atherosclerosis. (C) 2011 IUBMB IUBMB Life, 63(11): 987-1000, 2011″
“Background: Protothecosis is an uncommon human infection caused by Prototheca. Prototheca spp can be considered as saprophytes, and in spite of their frequency in the environment, they are of low virulence and may cause chronic infection with low-grade inflammation in humans. At present, only three species are recognized: Prototheca wickerhamii, Prototheca zopfii and Prototheca stagnora.

CCR6+CD146+ TEM (CD4+CD45RA-CCR7-) cells had a greater capacity to produce IL-17 than CCR6+CD146- or CCR6-CD146+ TEM cells. Although the percentage of CCR6+CD146+ cells in TEM cells was not significantly different between patients with psoriasis and controls, three of eight patients had a higher percentage of CCR6+CD146+ TEM cells than the mean +5 standard deviations of the controls. Coexpression

of CCR6 and CD146 is a useful marker for Th17EM cells. Increasing the number of CCR6+CD146+ Th17EM cells in peripheral blood may facilitate estimation of systemic Th17-cell activity in Th17-mediated diseases.”
“Background: Candida albicans has a variety of virulence factors, including secreted secondly aspartyl proteases, which are determinant

factors in the pathogenesis of this yeast in immunocompromised patients.\n\nAims: Proteinase activity was identified in C. albicans strains isolated from the oral cavity of immunocompromised patients selleck chemicals with cancer, diabetes and HIV+, with oral candidiasis and in healthy subjects.\n\nMethods: Two hundred and fifty C. albicans strains were analyzed, distributed in 5 different groups: patients with cancer, diabetes, HIV+, with oral candidiasis and healthy subjects.\n\nResults: Proteolytic activity was identified in 46% of the strains from cancer patients, 54% from HIV+ patients, 60% from diabetics, 70% from oral candidiasis patients, and 42% from healthy subjects. Activity was higher in strains from immunocompromised and

oral candidiasis patients than in healthy subjects. Differences were observed between the candidiasis-healthy, candidiasis-HIV+, and diabetic-healthy groups. No differences were observed between the oral candidiasis, diabetes and cancer patients, between the diabetes and HIV+ patients, or between the cancer patients, HIV+ patients and healthy subjects.\n\nConclusions: The present results suggest that although secreted aspartyl proteases are important in the pathogenesis of Brefeldin_A C albi cans, their activity depends on host conditions. (C) 2012 Revista lberoamericana de Micologia. Published by Elsevier Espaiia, S.L. All rights reserved.”
“The genetic variability of the brown pencilfish Nannostomus eques was studied, based on an analysis of sequences from the control region (1084 bp) of mitochondrial (mt)DNA in 125 individuals collected from eight tributaries along the upper (Acaituba, Miua, Jaradi and Arixana), middle (Demini), and lower (Jacunda Maguari and Catalao) Rio Negro (Brazil). Phylogenetic inferences using mtDNA data from N. eques revealed two evolutionary units. Genetic distance between them ranged from 5.5 to 8.3% and differed by 8.511.8% from the sister species pencilfish Nannostomus unifasciatus. The time of divergence between the two evolutionary units was estimated to be the Middle Pliocene (c. 2.