0 (The Metabolomics Innovation Centre (TMIC). Results: In all 42 metabolites were assigned in the proton NMR spectrum of patients with celiac disease. PLS-DA clearly differentiated patients with celiac disease from controls. A significantly higher concentration of lactate, pyruvate, succinate, fumarate, aspartate
and leucine were observed in the intestinal mucosa of patients with celiac disease than controls suggesting Ixazomib abnormalities in glycolysis and Kreb’s cycle (energy deficiency) and amino acid metabolism which may affect the biosynthetic pathways and consequently may contribute to villous abnormalities. Accumulation of aspartate indicated lower activity of aspartate
transaminase affecting its availability for urea cycle. Conclusion: Our data indicated characteristic metabonomic signature of small intestinal mucosa of patients with celiac disease. Key Word(s): 1. Celiac Disease; 2. Villous atrophy; 3. NMR spectroscopy; Presenting Author: WEIHONG TANG Additional Authors: QIAN XU, WENYU CONG Corresponding Author: QIAN XU, WENYU CONG Affiliations: General Hospital Y-27632 supplier of Armed Police Forces, Beijing; Department of Gastroenterology, Xijing hospital, The Fourth Military Medical University, Xi’an; Department of experimental therapy of Acute Radiation Sickness, Institute of Radiation Medicine, Academy of Military Medical Science, Beijing Objective: To establish a method of visualizing the microvascular system in the
small intestinal villi of mice by a cardiac perfusion of fluorescent dye DiI (1,1′-dioctadecyl – 3,3,3′3′ – tetramethylindocarbocyanine perchlorate) and to observe the villous microvascular changes MCE公司 in the pathogenesis of radiation enteritis (RE). Methods: C57BL/6 mice were irradiated with a single dose of γ rays to the abdomen to establish an animal model of RE. Radiation-induced intestinal mucosa damage in mice was examined by H&E staining. The blood vessels of mice were labeled by a cardiac perfusion of fluorescent dye DiI, and the labeled villous microvascular system, as well as its changes after irradiation, were observed by conventional and confocal fluorescence microscopy. Results: According to the pathological changes of the small intestine in irradiated mice, the method of visualizing blood vessels of mice by a cardiac perfusion of DiI was established. Complete and clear three-dimensional structure of the villous microvascular system of mice could be observed for the first time by fluorescence microscope or confocal laser scanning microscope thanks to DiI labeling.