Values shown are representative data from two independent experim

Values shown are representative data from two independent experiments. emhABC expression is affected by incubation temperature and growth phase Changes in the activity of EmhABC in cLP6a cells grown at different temperatures could reflect differential expression of emhABC, differential EmhABC translation or changes in the membrane physiology of the cells as a result of deviation

from the normal growth temperature. Thus we determined the effect of incubation temperature on the expression of emhABC and on the cell membrane physiology. It is assumed that the emhABC genes form an operon based on their homology to the ttgABC and mexAB-OprM efflux operons [18]. Expression learn more of the emhABC genes in cLP6a cells incubated at different temperatures and grown to different phases was determined using RT-qPCR to identify the condition(s) that induce emhABC transcription. The reference level of expression (i.e.,

calibrator) was defined as that exhibited by cLP6a cells grown to stationary phase at 28°C. Expression at 28°C was dependent on growth phase: emhABC genes were induced ~20-35 fold in log phase cells, and ~6-fold in death phase cells (Figure 3). Sub- and supra-optimal 4SC-202 molecular weight incubation temperature also increased expression ~10-fold at 10°C and ~32-fold at 35°C in stationary phase cells. The presence of tetracycline in the growth medium at 28°C induced emhABC by ~10-fold. Induction levels obtained for all these conditions were significantly different (P < 0.005) from the calibrator. In each case, except for logarithmic growth, the three emhABC genes were expressed at equivalent levels, but during log phase their expression followed the trend emhA > B > C. Figure 3 Expression of emhABC efflux genes. Expression of emhABC in P. Inositol monophosphatase 1 fluorescens strain cLP6a grown to stationary (Stat), logarithmic (Log) or Death phase at 28°C; grown to stationary phase

at 10°C or 35°C; grown to stationary phase at 28°C in the presence of chloramphenicol (Chl) or tetracycline (Tet) at 1/4 MIC; or grown to stationary phase at 28°C in the presence of naphthalene (Nap) or GANT61 chemical structure phenanthrene (Phen) at 5 mmol l-1, determined using RT-qPCR. The values shown are the fold-difference in expression of emhABC compared to expression levels in cells grown to stationary phase at 28°C (calibrator = 1). Each bar represents the mean of two independent experiments performed in duplicate. Error bars, where visible, indicate the average deviation. Expression of emhABC genes did not increase in stationary phase cells incubated at 28°C in the presence of chloramphenicol, naphthalene or phenanthrene although chloramphenicol and phenanthrene are known substrates of EmhABC efflux pump. This is consistent with the hypothesis that PAHs and antibiotics are not primary substrates of resistance-nodulation-division (RND) efflux pumps [6, 7]. The observation by Hearn et al.

Only organisms with completely sequenced genomes were chosen to a

Only organisms with completely sequenced genomes were chosen to avoid poor or incomplete sequence data from shotgun or partial genome sequencing projects. For each set of homologous matches,

there were four proteins: the duplicated genes and an ortholog match for each copy as only the best and most complete hits to each gene in a pair were selected. For these duplicate pairs, two alternative phylogenetic relationships were predicted. The Type-A relationship was predicted when a protein sequence CB-839 in vitro branched with a homolog (ortholog) from a closely related species rather than its counterpart protein (paralog) within the R. sphaeroides genome, whereas as Type-B relationship was predicted when the duplicate protein copies within R. sphaeroides branched with each other [28, 33]. Additionally, four example phylogenetic analyses, two exhibiting Type-A phylogeny GDC-0973 nmr and two exhibiting Type-B phylogeny, were carried out with gene duplications common among the four R. sphaeroides strains. Protein sequence alignments were carried out using MUSCLE [34], a program known for

its accuracy and speed. Phylogenetic Idasanutlin analysis was performed using PhyML [35] with the WAG model [36] to generate unrooted, maximum likelihood trees. Bootstrap values were calculated using 100 replications for the trees where topology was being determined. Maximum likelihood trees were constructed for all protein-pairs to ascertain the tree topology (Type-A or Type B). If a set of duplicated genes had their highest match to the same ortholog, then the next highest ortholog match, if available, for one of the genes was utilized in the tree construction Cell press to ascertain accurately the duplication topology. Functional Constraints Analysis For the functional constraints analysis, comparisons were conducted within all four R. sphaeroides strains. More specifically, the 28 common

gene pairs among the four strains were utilized for the functional constraints analysis where the genes in a given pair were compared against one another. The synonymous and nonsynonymous substitution rates along with the nonsynonymous-synonymous substitution rate ratio were calculated using the modified Yang-Nielsen algorithm [37, 38]. MUSCLE was used to align amino acid sequences [34]. These aligned sequences were then transformed into the original DNA sequences after which, the KaKs_Calculator was used with each pair of DNA sequences [39] to calculate the synonymous substitution rate (Ks), the nonsynonymous substitution rate (Ka), and the nonsynonymous/synonymous rate ratio (ω = Ka/Ks). Under the MYN model, ω = 0.3, 1, and 3 were used for negative (purifying), neutral, and positive selection, respectively [37, 38]. A one-way ANOVA was used to test whether the distributions of ω among the four strains were dissimilar.

The measured quantity of the mRNA in each of the treated samples

The measured quantity of the mRNA in each of the treated samples was normalized using the CT values obtained for the β-tubulin (Afu1g10910) mRNA amplifications run in the same plate. The relative quantitation of all the genes and tubulin gene expression was determined by a standard curve (i.e., CT -values plotted against logarithm of the DNA copy number). The results are the means ± standard deviation of four sets of experiments. The values represent the number of times the genes are expressed compared to the corresponding control strain grown before adding 200 mM CaCl2

(represented absolutely as 1.00). It is very impressive the mRNA accumulation levels of the Hsp9-12 heat shock protein Scf1 homologue 4EGI-1 in vivo (Afu1g17370): about 100 and 1000 times more in the ΔcrzA and ΔcalA than in the wild type, SRT2104 price respectively (Figure 1E). A. fumigatus has two Hsp12 homologues, Afu1g17370 (e-value = 3.7e-10; 45 and 57 identity and similarity, respectively) and Afu6g12450 (e-value = 3.1e-9; 39 and 56 identity and similarity, respectively). Interestingly, the S. cerevisiae HSP12 was also shown to be induced by calcium but in contrast to the A. fumigatus homologue, the S. cerevisiae gene is repressed when calcium+FK506 were added and accordingly repressed in the ΔCRZ1 background [30]. Thus, it remains to be determined the roles played by calcineurin, AfCrzA, and AfHsp12p during adaptation of A. fumigatus to calcium stress. Recently,

Hagiwara et al. [31] identified and characterized the A. nidulans AncrzA gene. They performed an in silico analysis by using MEME (Motif-based selleck chemicals llc sequence analysis tools; http://​meme.​sdsc.​edu/​meme4_​1_​1/​intro.​html) of the possible presence of a CDRE-like consensus motif in the promoter regions of 25 AnCrzA-dependent genes. By analyzing their promoter regions, 5′-G[T/G]GGC[T/A]G[T/G]G-3′

was presumed to be the consensus sequence for the A. nidulans AnCrzA-dependent genes. By using a combination of MEME analysis and the A. nidulans CDRE consensus as a guide, we were able to identify in the AfrcnA, AfrfeF, AfBAR, and the A. fumigatus phospholipase D promoter regions PI-1840 (about 500 bp upstream ATG) the following CDRE motifs: (i) AfrcnA (5′-GTTGGTGAG-3′, -314 bp upstream ATG starting point), (ii) AfrfeF (5′GTGGCTGAT-3′, -184 bp upstream ATG), (iii) AfBAR (5′-GTGGCTGAC-3′, -309 bp upstream ATG), and (iv) A. fumigatus phospholipase D (5′-GTTGGAGAG-3′, -239 upstream ATG). We compared these motifs with the promoter regions (about 500 bp upstream ATG) of 32 repressed genes described in Additional file 1, Table S1, and this analysis suggested 5′-GT[T/G]G[G/C][T/A]GA[G/T]-3′ as the CDRE-consensus sequence for A. fumigatus AfCrzA-dependent genes. We also analyzed Afscf1 and Af AAA ATPase genes and found the following CDRE-like motifs: (i) Afscf1 (5′-GGGAACGAA-3′, -376 bp upstream ATG), and (ii) Af AAA ATPase (5′-GAAGACGAG-3′, -19 bp upstream ATG).

In the present study, the production of Swiss Raclette type chees

In the present study, the production of Swiss Raclette type cheese with defined production and ripening parameters led to the development of a similar

flora in two distinct dairies. The source of this highly diverse flora remains unidentified but possible sources could be the brine bath, skin of the workers or wooden shelves, as shown by Mounier et al. [36] for Gubbeen cheese. The high biodiversity GF120918 is particularly surprising in the case of dairy F, where the smear brine is freshly prepared prior to each smearing and inoculated with a defined ripening culture of only 3 bacterial species. Moreover, the smear brine is applied by a cheese ripening robot that smears the young cheeses first. However, the microflora of the brine bath is not controlled and might be one of the

major sources. In particular, the brine bath (18-22% (w/v) NaCl) could be suitable to maintain the two halophilic and alkaliphilic marine LAB detected in consortium F, as some strains of M. psychrotolerans and Al. kapii were shown to grow at salt concentration as high as 21% (w/v) by Ishikawa et al. [37, 38]. Dynamic studies of consortia F and M inoculated at same cell counts on cheese surface revealed a similar sequential development of nine bacterial species, i.e. Lc. lactis, St. equorum, Al. kapii, C. casei, B. linens, BIBF 1120 purchase C. variabile, an uncultured selleckchem bacterium from marine sediment, (-)-p-Bromotetramisole Oxalate Mc. gubbeenense and Ag. casei. The development of this microbial community prevented growth of Listeria innocua, inoculated at 5 × 103 CFU ml-1 smear brine on cheeses at day 7 and 8, over 60 to 80 days ripening. Contamination at day 7 and 8, i.e. when yeasts reached their highest density, provided optimal growth conditions for Listeria, as shown by the rapid Listeria growth on control cheese. Strong antilisterial activities were shown in this unfavorable condition for consortia F and M. Antilisterial activities of

complex undefined cheese surface consortia were already observed in previous studies [9, 15]. Maoz et al. [9] reported a total inhibition of L. monocytogenens during 40 days ripening of a soft smear cheese with an initial contamination level of 1.6 × 103 CFU ml-1 smear brine. The surface of smear cheese contains a limited range of substrates supporting growth of microorganisms, mainly lactose and lactate. Lactose is mostly metabolized by LAB during curd acidification and initial ripening. The residual lactose can be metabolized on the cheese surface by yeasts during the first days of ripening, as shown for soft cheeses by Leclercq-Perlat et al. [39]. Lactate metabolized by yeasts into CO2 and H2O leads to deacidification of the cheese surface [40]. As a result, lactate continuously diffuses from the core to the surface of the cheese. Lactate can be totally consumed by surface microorganisms in soft cheeses [41].

Figure 2b shows the SEM image of the Au mesh film obtained after

Figure 2b shows the SEM image of the Au mesh film obtained after depositing the Au film on the patterned silicon (100) surface by ion check details sputtering (corresponding to Figure 1e). Due to the closure effect [13], the average apertures of the Au mesh decrease with increased thickness of the Au film. After depositing a 45-nm-thick Au film, the average hole diameter decreases to 65 nm ± 15%, as shown in Figure 2d. Figure 2 SEM image and diameter distribution of the patterned silicon surface and the Au mesh. (a) SEM image and (c) diameter distribution of the patterned silicon

surface after the removal of the AAO mask and SiO2 layer. (b) SEM image and (d) diameter distribution of the Au mesh after the deposition of the Au film on the patterned silicon surface. The bars in (c) and (d) represent the learn more measured statistical data, and the line is a Gaussian fitting. The sputtering

process resulted in a uniform deposition of the Au on the top surface of the patterned silicon, partially coating on the upper side walls, but not on the bottom of the holes, as shown BIRB 796 solubility dmso in Figure 3, which can be primarily attributed to the large depth-width ratio of the holes (approximately 5.6), considering the poor step coverage and the undemanding deposition conditions of ion sputtering. Figure 3 Cross-sectional SEM image of the patterned Si substrate covered with Au film. Structure of the SiNW arrays The resulting large-area, vertically aligned SiNW array is shown in Figure 4a. Upon close examination, Au can be clearly observed at the interfacial region between the SiNWs and the substrates, while no Au particle is found on the top of each SiNW. This result is consistent with the observation that Au is not deposited at the bottom of the holes (see Figure 3). Figure 4b shows that the SiNW exhibits a uniform diameter along the height direction, indicating that Au is inert against oxidative dissolution in the etching solution and is superior to the Ag catalyst which resulted in the tapered morphologies unless of the SiNWs with larger diameters at the bottom part due to the dissolution-induced gradual increase of the hole sizes of the Ag mesh during etching [12, 13]. Figure 4 Plan-view (a)

and cross-sectional (b) SEM images of the large-area, vertically aligned SiNW arrays. For the SEM observation, the sample was tilted by 15°. Effect of Au mesh thickness on the etching rate The Au films with thicknesses of 15, 30, and 45 nm were deposited on the same patterned Si substrate and then subjected to metal-assisted chemical etching for 10 min at 22°C. Interestingly, the height of the SiNWs catalyzed using a thick Au mesh was much larger compared with that catalyzed using a thin one (see Figure 5). The average heights of the resulting SiNWs are 220, 458, and 1,076 nm, respectively. Clearly, the disparity in the height of the SiNWs can be attributed to the different etching rates of the Si catalyzed using the Au meshes with different thicknesses.

The device size is 4 × 4 μm2 Every cycle data was captured durin

The device size is 4 × 4 μm2. Every cycle data was captured during measurement. The P/E voltages were +2/−2.2 V. Both HRS and LRS were read out at +0.1 V, and pulse width was 500 μs. The P/E cycles are not stable as we expected. Further study is needed to obtain stable P/E cycles. Long read pulse endurance of >106 cycles is shown in Figure 6b. In this case, stress pulse width was 500 μs and read pulse width was 10 μs. Stable LRS

is obtained at a V read of 0.1 V. Due to the strong Selleck SRT2104 conducting filament formation, stable LRS is observed under random read pulse. For LRS only, it took a long measurement time of approximately 3 days. On the other hand, the data retention is quite good after programming the device. The HRS was read out at two different V read’s of selleck screening library +0.1 and +0.05 V. Stable HRS is observed up to 400,000 cycles, and the HRS is decreased with pulse numbers. This may be due to defects creation during continuous AZD2171 mouse stress on the TaO x switching layer or the migration of oxygen ions

due to heating effects. Further study is needed to improve P/E endurance and instability of read pulse endurance of HRS after long cycles. However, a resistance ratio of >10 is obtained after 106 cycles. Our memory device also performs good data retention of >104 s as shown in Figure 7. The read voltage for both HRS and LRS was −0.2 V. An acceptable resistance ratio of >10 is observed after a retention time of DOCK10 104 s. This RRAM device is very useful for nanoscale non-volatile memory application. Figure 6 Endurance characteristics. (a) P/E

endurance of >103 cycles and (b) long read pulse endurance of >106 cycles of our novel W/TaO x /TiN memory device. The device size is 4 × 4 μm2. Figure 7 Data retention characteristics. Good data retention of >104 s of our W/TaO x /TiN memory device. An acceptable resistance ratio of >10 is obtained after 104 s. Conclusions One hundred consecutive switching cycles in the W/TaO x /TiN structures under self-compliance (<200 μA) and low-voltage operation of ±2.5 V are obtained. The thicknesses of TaO x and TiO x N y layers are 7 and 3 nm, respectively, which are observed by HRTEM. The RRAM device sizes are also confirmed by TEM. Our memory device shows good switching characteristics at low self-current compliance with tight distribution of HRS/LRS, excellent device-to-device uniformity, and program/erase endurance of >1,000 cycles. The smaller size devices show better switching characteristics and uniformity as compared to the larger size devices, owing to the thinner W electrode as well as higher series resistance. Interfacial oxygen-rich TaO x layer acts as a series resistance to control the resistive switching characteristics which may also cause the self-compliance resistive switching behavior and non-linear I-V curve at LRS. Switching mechanism is based on the formation and rupture of oxygen vacancy conducting path in the TaO x switching material.

Electrochem

Electrochem NVP-BSK805 cost FG-4592 mouse Commun 2012, 15:66–69.CrossRef 13. Gao P, Liu JC, Zhang T, Sun DD, Ng WJ: Hierarchical TiO 2 /CdS “spindle-like” composite with high photodegradation and antibacterial capability under visible light irradiation. J Hazard Mater 2012, 229–230:209–216.CrossRef 14. Liu BK, Wang DJ, Wang LL, Sun YJ, Lin YH, Zhang XQ, Xie TF: Glutathione-assisted hydrothermal synthesis of CdS-decorated TiO 2 nanorod arrays for quantum dot-sensitized solar cells. Electrochim Acta 2013, 113:661–667.CrossRef 15. Wu GS, Tian M, Chen AC: Synthesis of CdS quantum-dot sensitized TiO 2 nanowires

with high photocatalytic activity for water splitting. J Photoch Photobio A Chem 2012, 233:65–71.CrossRef 16. Xia MX, Wang FX, Wang YC, Pan AL, Selleck Vorinostat Zou BS, Zhang QL, Wang YG: TiO 2 nanowires sensitized with CdS quantum dots and the surface photovoltage properties. Mater Lett 2010, 64:1688–1690.CrossRef 17. Li X, Xia T, Xu CH, Murowchick J, Chen XB: Synthesis and photoactivity of nanostructured CdS-TiO 2 composite catalysts. Catal Today 2014, 225:64–73.CrossRef Competing interests The authors declare that they have no competing interests. Authors’ contributions YL and LZ prepared the films and tested the surface topography.

X-ray diffraction was investigated by PD and XY. The surface morphology and optical properties were measured by WW and GL. MW participated in the design and coordination of this study. The calculations were carried out by YL who also wrote the manuscript. All authors read and approved the final manuscript.”
“Background Binary transition metal oxides like NiO, TiO2, and ZnO have attracted much attention in the field of resistive switching due to simple constituents, low deposition temperature, and compatibility with complementary metal-oxide semiconductor technology

[1, 2]. Interestingly, different resistive switching behaviors have been found in metal/NiO/metal when different electrode materials were employed, such as Pt, Ag, Cu, and Al [3–6]. Lee et al. have found unipolar resistive switching (URS) in Ag(Cu)/NiO/Pt PRKACG due to the formation of an oxide layer at the metal/NiO interface [3]. Chiang et al. have demonstrated that bipolar resistive switching (BRS) in Al/NiO/indium tin oxide (ITO) as Al/NiO interfacial reaction region combined with ITO can form a dual-oxygen reservoir structure [4]. In addition, Ni/NiO/Ni with different device structure exhibits URS and BRS modes, separately driven by electrochemical- and thermal-based mechanisms [7]. Threshold resistive switching (TRS) and URS in NiO thin film were also found at different measuring temperatures by Chang et al.[8]. The occurrence of TRS and BRS in Mn-doped ZnO device was found with a higher CC by Yang et al. due to Joule heating [9].

AJR Am J Roentgenol 2000,175(6):1601–1607 PubMedCrossRef 15

AJR Am J Roentgenol 2000,175(6):1601–1607.PubMedCrossRef 15. Grassi R, Romano S, D’Amario F, et al.: The relevance of free fluid between intestinal loops detected by sonography in the clinical assessment of small bowel selleck kinase inhibitor obstruction in adults. Eur J Radiol 2004,50(1):5–14.PubMedCrossRef 16. Choi

HK, Chu KW, Law WL: Therapeutic value of gastrografin in adhesive small bowel obstruction after unsuccessful conservative treatment: a prospective randomized trial. Ann Surg 2002, 236:1–6.PubMedCrossRef 17. Srinivasa S, Thakore N, Abbas S, Mahmood M, Kahokehr AA, Hill AG: Impact of Gastrografin in clinical practice in the management of adhesive small bowel obstruction. Can PI3K Inhibitor Library price J Surg 2011.,54(2): 18. Wadani HAI, Al Awad NI, Hassan KA, Zakaria HM, Al Mulhim AA, Alaqeel FO: Role of water soluble contrast agents in assigning patients to a non-operative course in adhesive small bowel obstruction. Oman Med J 2011,26(6):454–456. doi:10.5001/omj.2011.116PubMedCrossRef 19. Di Saverio S, Catena F, Ansaloni L, Gavioli M, Valentino M, Pinna AD:

Watersoluble contrast medium (gastrografin) value in adhesive small intestine obstruction (ASIO): 4EGI-1 solubility dmso a prospective, randomized, controlled, clinical trial. World J Surg 2008,32(10):2293–2304.PubMedCrossRef 20. Barkan H, Webster S, Ozeran S: Factors predicting the recurrence of adhesive small-bowel obstruction. Am J Surg 1995, 70:361–365.CrossRef 21. Ivy L, Shing W, Wong P, Malouf P, Truskett G: Effect of handover on the outcomes of small bowel obstruction in an acute care surgery model. ANZ J Surg 2012. 10.1111/j.1445–2197.2012.06248.x 22. Leung AM, Vu H: Factors predicting need for and delay in surgery in small bowel obstruction. Am Surg 2012,78(4):403–407.PubMed 23. Fleshner PR, Siegman MG, Slater GI, Brolin RE, Chandler JC, Aufses AH Jr:

A prospective, randomized trial of short versus long tubes in adhesive small-bowel obstruction. Am J Surg 1995,170(4):366–370.PubMedCrossRef 24. Sakakibara selleck T, Harada A, Yaguchi T, Koike M, Fujiwara M, Kodera Y, Nakao A: The indicator for surgery in adhesive small bowel obstruction patient managed with long tube. Hepatogastroenterology 2007,54(75):787–790.PubMed 25. Moran BJ: Adhesion-related small bowel obstruction. Colorectal Dis 2007,9(Suppl 2):39–44.PubMedCrossRef 26. Fevang BT, Jensen D, Svanes K, Viste A: Early operation or conservative management of patients with small bowel obstruction? Eur J Surg 2002,168(8–9):475–481.PubMedCrossRef 27. Abbas S, Bissett IP, Parry BR: Oral water soluble contrast for the management of adhesive small bowel obstruction. Cochrane Database Syst Rev 2007,18(3):-CD004651. 28. Branco BC, Barmparas G, Schnüriger B, Inaba K, Chan LS, Demetriades D: Systematic review and meta-analysis of the diagnostic and therapeutic role of water-soluble contrast agent in adhesive small bowel obstruction. Br J Surg 2010,97(4):470–478.PubMedCrossRef 29.

Therefore, it seems that most (if not all) changes that could aff

Therefore, it seems that most (if not all) changes that could affect the functions of the encoded proteins have been removed by the action of purifying selection. Functional analysis of the nested consortium Most BMN 673 cost endosymbiotic systems analyzed to date at the genomic level have a nutritional basis, and many of them involve the biosynthesis of essential amino acids that are in short supply in the host diet. The metabolic pathways leading to amino acid biosynthesis in the T. princeps-M. selleck chemicals llc endobia consortium found in P. citri were recently analyzed in detail by McCutcheon and von Dohlen [16] and, therefore, they will

not be dealt with in this study. These authors also stated that T. princeps is unable to perform DNA replication, recombination or repair by itself, and the same applies to translation. They speculate that a passive mechanism such as cell lysis could provide T. princeps with the needed gene products from M. endobia. Our present work provides a detailed analysis of the M. selleck chemicals endobia functional capabilities, based on a functional analysis of its genome, regarding informational

functions or other intermediate metabolism pathways beyond amino acids biosynthesis. In the following sections these functional capabilities will be analyzed in a comprehensive manner, considering both endosymbiotic partners, in order to identify putative additional levels of complementation between them. DNA repair and recombination Contrary to what is found in bacterial endosymbionts with similarly

reduced genomes, M. endobia has quite a complete set of genes for DNA repair and recombination, while none were annotated in the T. princeps genome [16, 19]. Although it has lost the nucleotide excision repair genes (only uvrD is present), M. endobia retains a base excision repair system (the DNA glycosylases encoded by mutM and ung plus xth, the gene encoding exonuclease III, involved in the repair of sites where damaged bases have been removed). The mismatch repair system is also almost complete, since only mutH, encoding the endonuclease needed in this process to cleave the unmethylated strand, has been lost. Additionally, M. endobia also retains almost the entire molecular machinery for homologous recombination (recABCGJ, ruvABC, priAB), which Mirabegron could be responsible for the concerted evolution of the duplications in both genomes. In the absence of recD, the RecBC enzyme can still promote recombination, since it retains helicase and RecA loading activity. The missing exonuclease V activity can be replaced by other exonucleases with ssDNA degradation activity in the 5′ → 3′ sense, such of RecJ [30], which has been preserved. The final step in homologous recombination requires the reloading of origin-independent replication machinery. Two replisome reloading systems have been described in E.

2012R1A1A2004366 and (MSIP) No 2014R1A1A1005901 This work was al

2012R1A1A2004366 and (MSIP) No.2014R1A1A1005901. This work was also supported by a Research Grant of Kwangwoon University in 2014. Also, we would like to thank Mr. Ho-Kun Sung from Korea Advanced Nano Fab Center (KANC) for his technical support with the materials and circuit fabrications during this work. References 1. Wang C, Lee WS, Kim NY: Practical integrated passive device technology on GaAs. Microwave J 2012, 55:94–106. 2. Wang C, Zhang

F, Kim NY: Development and characterization of metal-insulator-metal capacitors with SiNx thin films by plasma-enhanced chemical vapor deposition. Chinese Phys Lett 2010, 27:078101. 10.1088/0256-307X/27/7/078101CrossRef 3. Wang C, Lee WS, Zhang F, Kim NY: A novel method for the fabrication of integrated passive devices on SI-GaAs substrate. Int J Aav Manuf Tech 2011, 52:1011–1018. 10.1007/s00170-010-2807-zCrossRef HMPL-504 manufacturer 4. Robutel R, Martin C, Buttay C, Morel H, Mattavelli P, Boroyevich D, Meuret R:

Design and implementation of integrated common mode capacitors for SiC JFET inverters. IEEE T Power Electr 2013, 29:3625–3636.CrossRef 5. Wang C, Kim NY: Electrical characterization and nanoscale surface morphology of optimized Ti/Al/Ta/Au ohmic contact for AlGaN/GaN HEMT. Nanoscale Res Lett 2012, 7:1–8. 10.1186/1556-276X-7-1CrossRef 6. Ramadass YK, Fayed AA, Chandrakasan AP: A fully-integrated switched-capacitor step-down DC-DC converter with digital capacitance modulation in 45 nm CMOS. IEEE J Solid-ST Circ 2010, 45:2557–2565.CrossRef 7. Naghib-Zadeh H, Glitzky C, Oesterle W, Rabe T: Low Selleck BYL719 temperature sintering of barium MM-102 concentration titanate based ceramics with high dielectric constant for LTCC applications.

J Eur Ceram Soc 2011, 31:589–596. 10.1016/j.jeurceramsoc.2010.10.003CrossRef 8. Saravanan KV, Raju KCJ: Quasi-rapid thermal annealing studies on barium strontium titanate thin films deposited on fused silica substrates. J Alloy Compd 2013, 571:43–49.CrossRef 9. Akedo J, Lebedev M: Aerosol deposition method (ADM): a novel method of PZT thick films producing Thiamet G for microactuators. Recent Res Dev Mater Res 2001, 2:51–77. 10. Kim HK, Oh JM, Kim SI, Kim HJ, Lee CW, Nam SM: Relation between electrical properties of aerosol-deposited BaTiO 3 thin films and their mechanical hardness measured by nano-indentation. Nanoscale Res Lett 2012, 7:1–8. 10.1186/1556-276X-7-1CrossRef 11. Oh JM, Nam SM: Thickness limit of BaTiO 3 thin film capacitors grown on SUS substrates using aerosol deposition method. Thin Solid Films 2010, 518:6531–6536. 10.1016/j.tsf.2010.03.159CrossRef 12. Oh JM, Nam SM: Role of surface hardness of substrates in growing BaTiO 3 thin films by aerosol deposition method. Jpn J Appl Phys 2009, 48:09KA07. 13. Oh JM, Kim NH, Choi SC, Nam SM: Thickness dependence of dielectric properties in BaTiO 3 films fabricated by aerosol deposition method. Mat Sci Eng: B 2009, 161:80–84. 10.1016/j.mseb.2009.01.028CrossRef 14.