J Nanotechnology 2012, 2012:1181–1184. 46. Dato A, Radmilovic V, Lee Z, Phillips J, Frenklach M: Substrate-free gas-phase synthesis of graphene sheets. Nano Lett 2012–2016, 2008:8. 47. Lee S, Lee K, Zhong Z: Wafer scale homogeneous bilayer graphene films by chemical vapor deposition. Nano Lett 2010, 10:4702–4707.CrossRef 48. Lee C, Li QY, Kalb W, Liu XZ, Berger

H, Carpick RW, Hone J: Frictional characteristics of atomically thin sheets. Science 2010, 328:76–80.CrossRef 49. Zhi C, Bando Y, Tang C, Kuwahara H, Golberg D: Large-scale fabrication of boron nitride nanosheets and their utilization in polymeric composites with improved thermal and mechanical properties. Adv Mater 2009, 21:2889–2893.CrossRef 50. Coleman JN, Lotya M, O’Neill A, Bergin SD, King PJ, Khan U, Young K, Gaucher A, De S, Smith RJ, Shvets BGB324 in vivo PF-562271 in vitro IV, Arora SK, Stanton G, Kim H-Y, Lee K, Kim GT,

Duesberg GS, Hallam T, Boland JJ, Wang JJ, Donegan JF, Grunlan JC, Moriarty G, Shmeliov A, Nicholls RJ, Perkins JM, Grieveson EM, Theuwissen K, McComb DW, Nellist PD, et al.: Two-dimensional nanosheets produced by liquid exfoliation of layered materials. Science 2011, 331:568–571.CrossRef 51. Nicolosi V, Chhowalla M, Kanatzidis MG, Strano MS, Coleman JN: Liquid exfoliation of layered materials. Science 2013, 340:1420–1424.CrossRef Competing interests The authors declare that they have no competing interests. Authors’ contributions VS was the main author of the work, performed the syntheses, and coordinated all characterization. Dichloromethane dehalogenase MS was responsible

for the electron microscopy, JH for the AFM microscopy, and PE for the XRD measurement. All authors read and approved the final manuscript.”
“Background The common stabilizers used in bottom-up approach of metallic nanoparticle synthesis (Au, Pt, Ag, etc.) are polymers and surfactants such as sodium dodecyl sulfate (SDS) and Tween 80 [1]. Furthermore, citrate which is a typical electro-static stabilizer has been popularly used [2]. Followed by citrate, the synthetic polymers have also been widely used as stabilizers, typically polyvinyl alcohol (PVA) [3–5] and polyvinyl pyrrolidone (PVP) [5–9]. On the other hand, among the natural polysaccharide stabilizers, chitosan [10–14] and alginate [15, 16] are commonly used. In addition, the derivatives of cellulose such as carboxylmethyl cellulose [1, 17], hydroxypropyl cellulose [18], and gelatin [7, 19] were also used as stabilizers for the synthesis of metallic nanoparticles. The study results of Kvítek et al. showed that the antibacterial activity of silver nanoparticles (AgNPs) was significantly enhanced by using the suitable stabilizers such as the following surfactants: SDS, Tween 80, and PVP K90 [1]. Moreover, the study results of El Badawy et al.

PubMed 2. Lin J, Lee IS, Frey J, Slonczewski JL, Foster JW: Comparative analysis of extreme acid survival in Salmonella typhimurium, Shigella flexneri, and Escherichia coli. J Bacteriol this website 1995,177(14):4097–4104.PubMed 3. Murphy C, Carroll C, Jordan KN: Induction of an adaptive tolerance response in the foodborne pathogen, Campylobacter jejuni. FEMS Microbiol Lett 2003,223(1):89–93.PubMedCrossRef 4. Smibert RM: The genus

Campylobacter. Annu Rev Microbiol 1978, 32:673–709.PubMedCrossRef 5. Audia JP, Webb CC, Foster JW: Breaking through the acid barrier: an orchestrated response to proton stress by enteric bacteria. Int J Med Microbiol 2001,291(2):97–106.PubMedCrossRef 6. Rao KA, Yazaki E, Evans DF, Carbon R: Objective evaluation of small bowel and colonic transit time using pH telemetry in athletes with gastrointestinal symptoms. Br J Sports Med 2004,38(4):482–487.PubMedCrossRef 7. Baik HS, Bearson S, Dunbar S, Foster JW: The acid tolerance response of Salmonella typhimurium provides protection against organic acids. Microbiology 1996,142(Pt 11):3195–3200.PubMedCrossRef 8. Cotter PD, Gahan CG, Hill C: Analysis of the role of the Listeria monocytogenes F0F1 -AtPase

operon in the acid tolerance response. Int J Food Microbiol 2000,60(2–3):137–146.PubMedCrossRef 9. Schneider E, Altendorf K: Bacterial adenosine 5′-triphosphate synthase (F1F0): purification and reconstitution of F0 complexes and biochemical and functional characterization CX-5461 in vitro of their subunits. Microbiol Rev 1987,51(4):477–497.PubMed 10. Merrell DS, Camilli A: The cadA gene of Vibrio cholerae is induced during infection and plays a role in acid tolerance. Mol Microbiol why 1999,34(4):836–849.PubMedCrossRef 11. Park YK, Bearson B, Bang SH, Bang IS, Foster JW: Internal pH crisis, lysine decarboxylase and the acid tolerance response of Salmonella typhimurium. Mol Microbiol 1996,20(3):605–611.PubMedCrossRef 12. Richard HT, Foster JW: Acid resistance in Escherichia coli. Adv Appl Microbiol 2003, 52:167–186.PubMedCrossRef 13. Parkhill J, Wren BW, Mungall K, Ketley JM, Churcher

C, Basham D, Chillingworth T, Davies RM, Feltwell T, Holroyd S, Jagels K, Karlyshev AV, Moule S, Pallen MJ, Penn CW, Quail MA, Rajandream MA, Rutherford KM, van Vliet AH, Whitehead S, Barrell BG: The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences. Nature 2000,403(6770):665–668.PubMedCrossRef 14. Magnusson LU, Farewell A, Nystrom T: ppGpp a global regulator in Escherichia coli. Trends Microbiol 2005,13(5):236–242.PubMedCrossRef 15. Foster JW: Escherichia coli acid resistance: tales of an amateur acidophile. Nat Rev Microbiol 2004,2(11):898–907.PubMedCrossRef 16. Lee IS, Lin J, Hall HK, Bearson B, Foster JW: The stationary-phase sigma factor sigma S (RpoS) is required for a sustained acid tolerance response in virulent Salmonella typhimurium. Mol Microbiol 1995,17(1):155–167.PubMedCrossRef 17.

It is possible that some kinds of cell growth or division signals are misread in the presence of phenol in the

colR mutant, which eventually leads to the cell lysis. In that case phenol could act as a signal, leading to the cell death, rather than being killing factor itself. Our further experiments will hopefully clarify whether phenol- and glucose-caused stresses originate from the same defect of the colR mutant or they are caused by different reasons. Conclusions Current study demonstrates the involvement of the ColRS two-component system and the TtgABC efflux pump in phenol tolerance of P. putida. Our results imply that TtgABC and ColRS systems are not directly connected find more and may affect phenol tolerance via independent pathways. Both these systems affect phenol tolerance of growing cells only but not of starving ones, indicating that ColRS and TtgABC systems affect processes occurring in metabolically active and dividing bacteria. Most tolerance mechanisms to aromatic hydrocarbons are directed toward maintaining the cell membrane intactness [2]. Given that ColRS and TtgABC systems are also implicated in membrane functions [12, 30, 38], it is reasonable to conclude that they may assist in regulation of biosynthesis and/or turnover

of membrane components, so helping to maintain membrane homeostasis during growth and division. Population structure analysis at single cell level revealed that strong cell division inhibition occurred in phenol-exposed population which LEE011 supplier could be considered as adaptive response to phenol stress to reduce the phenol-caused damage and to maintain membrane homeostasis. Acknowledgements We are grateful to Tiina Alamäe and Paula Ann Kivistik for critically reading the manuscript. We thank Riho Teras for plasmid pUCNotKm. Dimitri Lubenets is specially acknowledged for operating FACSAria. This work was supported by grant 7829 from the Estonian Science Foundation to R. H., and by funding of Targeted Financing Project TLOMR0031 from the Estonian Ministry of Research and Education and by grant HHMI 55005614 from the Howard Hughes

Medical CHIR-99021 order Institute International Research Scholars Program to M. K. Electronic supplementary material Additional file 1: Plate assay of phenol tolerance of P. putida PaW85 (wt) and colR -deficient (colR) strains. Cells were grown on glucose (glc) minimal medium in the presence or absence of 8 mM phenol. Approximate number of inoculated bacterial cells is indicated above the figure. Bacteria were photographed after 4 days of growth. (PDF 188 KB) Additional file 2: Comparative analysis of subpopulations with different DNA content by staining of cells with SYTO9 and PI or SYTO9 alone. P. putida wild-type (wt) and ttgC-deficient (ttgC) strains were grown for 24 h on gluconate minimal plates supplemented with 8 mM phenol. Cells were stained with PI and SYTO9 (SYTO9+PI) or SYTO9 alone and analysed by flow cytometry.

Ling et al. reported MG-132 nmr that despite SOX9 levels being high during periods of prenatal urothelial development in mouse bladders, SOX9 was diminished and quiescent with maturation after birth, but was rapidly induced by a variety of injuries and urothelial cancer [19]. All these findings

suggest that SOX9 may play important roles in cancer development and progression, which prompted the authors to ask whether it is also clinically associated with the progression of NSCLC. To address this question, studies were performed to characterize the expression of SOX9 in NSCLC cell lines and clinical lung cancer tissues. The data show that upregulation of SOX9 mRNA and protein is a common and frequent event in both NSCLC cell lines and human lung cancer tissues. Comparative analyses of SOX9 mRNA and protein in lung cancer tissues and their paired adjacent normal tissue have provided strong support for the identified upregulation of

SOX9 in NSCLC. Moderate to strong cytoplasmic staining of SOX9 was displayed in tumor cells from 135/142 (95.1%) paraffin-embedded archived NSCLC biopsy samples in comparison with the adjacent non-cancerous cells, which expressed little, if any, SOX9. Further analysis of the relationship between SOX9 staining and the clinicopathological characteristics of patients showed a significant correlation between SOX9 expression and the histopathological staging of NSCLC. This revealed that SOX9 Selleck p38 MAPK inhibitor levels were higher in advanced stages of the disease, supporting the hypotheses that SOX9 may play a role in the progression of NSCLC and that it could represent a biomarker that identifies subsets of lung-cancer patients with more aggressive disease. It is of particular note that patients with high SOX9 expression had shorter survival time, suggesting the possibility of using SOX9 as a predictor for patient prognosis and survival. In a more detailed survival study, univariate and multivariate analyses

demonstrated that high expression of SOX9 is a predictor of poor prognosis for lung-cancer patients. It is of note that there is a significant correlation between shorter overall survival times of patients and high SOX9 expression in both the early histological stage subgroup Dichloromethane dehalogenase (stages I and II) and the late histological stage subgroup (stages III and IV), suggesting that SOX9 may be a useful prognostic marker for all stages of NSCLC. Conclusions Although several lines of evidence have suggested that SOX9 might be involved in cancer development and progression, only a few studies have linked SOX9 to lung cancer. Knockdown of SOX9 has been found to decrease the proliferation rate of lung cancer cell lines and significantly attenuate the tumorigenicity of lung adenocarcinoma [6]. Despite the above finding, the precise pathway that SOX9 uses to inhibit the differentiation of NSCLC and promote lung cancer development and progression remains unclear.

PubMed 12. Brismar B, Edlund C, Malmborg AS, Nord CE: Ciprofloxacin concentrations and impact of the colon microflora in patients undergoing colorectal surgery. Antimicrob Agents Chemother 1990,34(3):481–483.PubMedCrossRef 13. Condon RE, Walker AP, Hanna CB, Greenberg RN, Broom A, Pitkin D: Penetration of meropenem in plasma and abdominal tissues from patients undergoing intraabdominal surgery. Clin Infect Dis 1997,24(Suppl 2):S181-S183.PubMedCrossRef learn more 14. Wong CS, Jelacic S, Habeeb RL, Watkins SL, Tarr PI: The risk of the hemolytic-uremic syndrome after antibiotic treatment of Escherichia coli O157:H7 infections. N Engl J Med 2000,342(26):1930–1936.PubMedCrossRef

15. Safdar N, Said A, Gangnon RE, Maki DG: Risk of hemolytic uremic syndrome

after antibiotic treatment of Escherichia coli O157:H7 enteritis: a meta-analysis. Jama 2002,288(8):996–1001.PubMedCrossRef 16. Martin DL, MacDonald KL, White KE, Soler JT, Osterholm MT: The epidemiology and clinical aspects of the hemolytic uremic syndrome in Minnesota. N Engl J Med 1990,323(17):1161–1167.PubMedCrossRef 17. Bell BP, Griffin PM, Lozano P, Christie DL, Kobayashi JM, Tarr PI: Predictors of hemolytic uremic syndrome in children Maraviroc chemical structure during a large outbreak of Escherichia coli O157:H7 infections. Pediatrics 1997,100(1):E12.PubMedCrossRef 18. Ikeda K, Ida O, Kimoto K, Takatorige T, Nakanishi N, Tatara K: Effect of early fosfomycin treatment on prevention of hemolytic uremic syndrome accompanying Escherichia coli O157:H7 infection. Clin Nephrol 1999,52(6):357–362.PubMed 19. Kurioka T, Yunou Y, Harada H, Kita E: Efficacy of antibiotic therapy for infection with Shiga-like

toxin-producing Escherichia coli O157:H7 in mice with protein-calorie malnutrition. Eur J Clin Microbiol Infect Dis 1999,18(8):561–571.PubMedCrossRef 20. Rahal EA, Kazzi N, Kanbar A, Abdelnoor AM, Matar GM: Role of rifampicin in limiting Escherichia coli O157:H7 Shiga-like toxin expression and enhancement of survival of infected BALB/c mice. Int J Antimicrob Agents 2011,37(2):135–139.PubMedCrossRef 21. Rahal EA, Kazzi N, Sabra A, Abdelnoor AM, Matar GM: Decrease in Shiga toxin expression using a minimal inhibitory concentration of rifampicin selleck chemical followed by bactericidal gentamicin treatment enhances survival of Escherichia coli O157:H7-infected BALB/c mice. Ann Clin Microbiol Antimicrob 2011, 10:34.PubMedCrossRef 22. Bielaszewska M, Idelevich EA, Zhang W, Bauwens A, Schaumburg F, Mellmann A, Peters G, Karch H: Epidemic Escherichia coli O104:H4: Effects of antibiotics on Shiga toxin 2 production and bacteriophage induction. Antimicrob Agents Chemother 2012,56(6):3277–3282.PubMedCrossRef 23. Sharma VK, Dean-Nystrom EA, Casey TA: Semi-automated fluorogenic PCR assays (TaqMan) forrapid detection of Escherichia coli O157:H7 and other shiga toxigenic E. coli. Mol Cell Probes 1999,13(4):291–302.PubMedCrossRef 24. Gentry MK, Dalrymple JM: Quantitative microtiter cytotoxicity assay for Shigella toxin. J Clin Microbiol 1980,12(3):361–366.

As noted by Lacroix et al. [18], given the weak magnetic fields in hyperthermia treatment, the maximum SAR would be obtained for soft ferromagnetic nanoparticles or the nanoparticles near the superparamagnetic transition. This is consistent with our experimental results. Conclusions Size-controlled synthesis of FeCo nanoparticles was done using selleck microemulsion method. It was observed that by increasing the water-to-surfactant molar ratio, the nanoparticles become

larger. The maximum size of nanoparticles in the ternary system of water/CTAB/hexanol is about 7 nm. Size dependency of magnetic properties including M s and H c was investigated. The observed increase in M s with size is due to disappearance of the magnetic dead layer in larger nanoparticles. However, the observed change in coercivity with size is due to transition between various size regimes and consequently the magnetization reversal mechanisms. The nanoparticles were stabilized using a CTAB/1-butanol bilayer. The stability of nanoparticles was studied at various nanoparticle sizes and concentrations. Results show that by increasing the nanoparticle size or concentration, the stability of

the magnetic fluid decreases due to magnetic interaction and consequent aggregation of nanoparticles. The inductive properties of nanoparticles OSI-906 manufacturer such as temperature rise and specific absorption rate were evaluated at various nanoparticle sizes and were observed to have direct relation with the size of nanoparticles. Both H c and SAR show similar tendencies of changing with particle size. The reason lies in anisotropy as a central parameter controlling both H c and SAR. Only W4 and W3 ferromagnetic nanoparticles are found to be capable of being used in hyperthermia treatment which passed the minimum temperature rise of 5°C to 9°C. The comparison of experimental results with those of Stoner-Wohlfarth and LRT models shows that hysteresis and relaxation mechanisms are both involved in the generation of heat, but the contribution of hysteresis is far greater than relaxation. Acknowledgement

The authors would like to thank Mr. B. Saberi for his great Etofibrate help in providing the requested facilities of this work. References 1. Hong RY, Li JH, Li HZ, Ding J, Zheng Y, Wei DG: Synthesis of Fe 3 O 4 nanoparticles without inert gas protection used as precursors of magnetic fluids. J Magn Magn Mater 2008, 320:1605–1614.CrossRef 2. Suresh G, Saravanan P, Babu DR: One-pot synthesis of Fe-Co nanospheres by modified polyol process and their structural, magnetic studies. J Phys, Conference Series 2011, 292:012015.CrossRef 3. Feng B, Hong RY, Wang LS, Guo L, Li HZ: Synthesis of Fe/APTES/PEG diacid functionalized magnetic nanoparticles for MR imaging. Colloid Surf A 2008, 328:52–59.CrossRef 4.

majuscula (L8106_07471, L8106_07436, L8106_07426, L8106_07421 and L8106_07416, respectively). Upstream of hoxE, the protein encoded by the partially sequenced ORF13 contains a pyruvate flavodoxin/ferredoxin oxidoreductase domain. The gene immediately downstream of hoxH, ORF 14, encodes a protein containing three transmembrane α-helices predicted by TMHMM2.0 http://​www.​cbs.​dtu.​dk/​services/​TMHMM/​. ORF14 also shows homology to cyanobacterial genes coding for putative membrane proteins. The following genes, named xisH and xisI, have homologues in several cyanobacterial strains, and although it has been demonstrated that they are required for the heterocyst-specific

excision of the fdxN element (fdxN encodes a heterocyst-specific ferredoxin) in Nostoc sp. PCC 7120 [27], they have been found in several unicellular and nonheterocystous TSA HDAC strains, as in Sorafenib in vivo the case of L. majuscula. In the nonheterocystous strains the function of the proteins encoded by xisH and xisI

is still to be disclosed. The three ORFs identified downstream of hoxW, have homologues in other cyanobacterial genomes, nevertheless the function of the encoded proteins is not known. Putative hydrogenase-specific endopeptidases genes and proteins In L. majuscula, the genes encoding the putative hydrogenase-specific endopeptidases, hoxW and hupW, are in the vicinity of the respective hydrogenases structural genes as it is common for cyanobacteria [3, 15–18]. The deduced 152 amino acid sequence of L. majuscula HoxW shows homology with the corresponding sequences of cyanobacteria with values varying between 32% and 82% of identity. In contrast, the SSR128129E deduced amino acid sequence of HupW from L. majuscula shows 59% to 80% of identity compared to the corresponding cyanobacterial sequences, being overall much less variable than HoxW. HoxW and HupW from L.

majuscula exhibit only 23% identity between themselves, a range that is frequent for other cyanobacterial strains. This low homology might be related to the specifiCity of the endopeptidases towards the hydrogenases large subunits, a subject that needs further investigation. Promoter regions and transcription of the hox genes In L. majuscula, hoxEF-hcp-hoxUYH are transcribed as an operon, as it could be predicted by the physical organization of the genes in a single cluster. In agreement with the different patterns of organization, the cyanobacterial hox genes can be transcribed as one or several units depending on the strain [15, 16, 18, 28–30]. L. majuscula hoxW, is not cotranscribed with the bidirectional hydrogenase structural genes or ORF14 but it is transcribed together with the four ORFs immediately upstream (xisH, xisI, ORF15 and ORF16), and its transcription is most probably controlled by the xisH promoter.

Physica B 2001, 298:472.CrossRef 33. Morkoç H, Uzgür U: Zinc Oxide, Fundamentals. New York, Wiley: Materials and Device Technology; 2009.CrossRef

Competing interest The authors declare that they have no competing interests. Authors’ contributions MZ and CK carried out the synthesis, scanning electron microscopy and X-ray diffraction. The optical properties were measured by AO. The calculations were carried out by MZ who was also wrote the manuscript. All authors read and approved the final manuscript.”
“Background Attractive interdisciplinary research areas between electronic and photonic materials have been developed by modern semiconductor nanotechnology. Si nanostructures are particularly important because solar cells using Si have widely been investigated [1, 2], and optical interconnections among integrated Si circuits have also been proposed by developing Si-based photodiodes and optical modulators www.selleckchem.com/products/r428.html [3, 4]. Therefore, many types of Si nanostructures, such as nanocrystals (NCs), nanodots, and PD0325901 ic50 porous nanostructures, were reported by employing various fabrication processes [5–14]. Moreover, fabrication processes of the Si nanostructures using ‘top-down’ lithography techniques were strongly motivated for the purpose of applying the Si nanostructures to electronic

and photonic devices. We have recently proposed a fabrication process of Si nanodisk (ND) arrays, where the Si NDs are formed by damage-free neutral beam (NB) etching for Si thin films covered with etching masks of Fe

nanoparticles which are regularly aligned by bio-protein engineering [15–20]. This fabrication process using the bio-templates enables us to prepare closely packed high-density Si RVX-208 NDs with the intentionally designed precise size and spacing in a nanometric scale with flexible film stacking. We have also observed intense photoluminescence (PL) emissions in a visible light region with fast decay times ranging from 10 ps to 2 ns [20]. The fast decaying PL characteristics reflect the dynamics of photo-excited carriers in this high-density Si ND array system, in which wavefunctions of photo-excited carriers overlap among Si NDs to some extent, and the carriers can transfer among the NDs [20]. Photo-generated or electrically injected carriers need to be effectively transferred among Si NDs for the optical applications to solar cells or light-emitting diodes. The spatial transfer of the carriers in nanostructures can also be affected by thermal effects, such as thermal hopping or escape. Therefore, in this paper, we investigate the detailed temperature dependence of time-resolved PL and the related carrier dynamics in these high-density Si ND arrays. Different types of PL quenching mechanism can be identified, and the activation energies for the PL thermal quenching are deduced from the temperature dependences of the PL intensity.

PubMedCrossRef 19. Leiby DA, Chung AP, Cable RG, Trouern-Trend J, McCullough J, Homer MJ, Reynolds LD, Houghton RL, Lodes MJ, Persing DH: Relationship between tick bites and the seroprevalence of Babesia microti and Anaplasma phagocytophila (previously Ehrlichia sp.) in blood donors. Transfusion 2002,42(12):1585–1591.PubMedCrossRef 20. Sweeney CJ, Ghassemi M, Agger WA, Persing DH: Coinfection with Babesia microti and Borrelia burgdorferi in a western Wisconsin resident. Mayo Clin Proc 1998,73(4):338–341.PubMedCrossRef 21. Mitchell PD, Reed KD, Hofkes JM: Immunoserologic evidence of coinfection

with Borrelia burgdorferi, Babesia microti, and human granulocytic Ehrlichia species in residents of Wisconsin Deforolimus manufacturer and Minnesota. J Clin Microbiol 1996,34(3):724–727.PubMedCentralPubMed 22. Chandrashekar R, Mainville CA, Beall MJ, O’Connor

T, Eberts MD, Alleman AR, Gaunt SD, Breitschwerdt EB: Performance of a commercially available in-clinic ELISA for the detection of antibodies against Anaplasma phagocytophilum, Ehrlichia canis, and Borrelia burgdorferi and Dirofilaria immitis antigen in dogs. Am J Vet Res 2010,71(12):1443–1450.PubMedCrossRef 23. Ravnik U, Tozon N, Smrdel KS, Zupanc TA: Anaplasmosis in dogs: the relation of haematological, biochemical and clinical alterations to antibody titre and PCR confirmed infection. Vet Microbiol 2011,149(1–2):172–176.PubMedCrossRef 24. Herwaldt BL, Linden JV, Bosserman E, Young C, Olkowska D, Wilson M: Transfusion-associated babesiosis in the United States: a description of cases. Ann Intern Med 2011,155(8):509–519.PubMedCrossRef 25. Hatcher JC, Greenberg PD, Antique J, Jimenez-Lucho VE: Severe babesiosis in Long Island: review of 34 cases and their complications. Target Selective Inhibitor Library Clin Infect Dis 2001,32(8):1117–1125.PubMedCrossRef 26. Summary of notifiable diseases — United States, 2009 MMWR Morb Mortal Wkly Gemcitabine cost Rep 2011,58(53):1–100. 27. Wormser GP, Aguero-Rosenfeld ME, Cox ME, Nowakowski J, Nadelman RB, Holmgren D, McKenna D, Bittker S, Zentmaier L, Cooper D, et al.: Differences and

similarities between culture-confirmed human granulocytic anaplasmosis and early Lyme disease. J Clin Microbiol 2013,51(3):954–958.PubMedCentralPubMedCrossRef 28. Chmielewska-Badora J, Moniuszko A, Zukiewicz-Sobczak W, Zwolinski J, Piatek J, Pancewicz S: Serological survey in persons occupationally exposed to tick-borne pathogens in cases of co-infections with Borrelia burgdorferi, Anaplasma phagocytophilum, Bartonella spp. and Babesia microti . Ann Agric Environ Med 2012,19(2):271–274.PubMed 29. Lommano E, Bertaiola L, Dupasquier C, Gern L: Infections and coinfections of questing Ixodes ricinus ticks by emerging zoonotic pathogens in Western Switzerland. Appl Environ Microbiol 2012,78(13):4606–4612.PubMedCentralPubMedCrossRef 30. Franke J, Hildebrandt A, Meier F, Straube E, Dorn W: Prevalence of Lyme disease agents and several emerging pathogens in questing ticks from the German Baltic coast. J Med Entomol 2011,48(2):441–444.PubMedCrossRef 31.

Urwin R, Maiden MCJ: Multi-locus sequence typing: a tool for global epidemiology. Trends Microbiol 2003, 11:479–487.PubMedCrossRef 16. Meinersmann R, Phillips R, Wiedmann M, Berrang M: Multilocus sequence typing of Listeria monocytogenes by use of hypervariable genes reveals clonal and recombination histories of three Y-27632 purchase lineages. Appl Environ Microbiol 2004, 70:2193–2203.PubMedCrossRef 17. Chen J, Luo X, Jiang L, Jin P, Wei W, Liu D, Fang W: Molecular characteristics and virulence

potential of Listeria monocytogenes isolates from Chinese food systems. Food Microbiol 2009, 26:103–111.PubMedCrossRef 18. Glaser P, Frangeul L, Buchrieser C, Rusniok C, Amend A, Baquero F, Berche P, Bloecker H, Brandt P, Chakratory T, Charbit A, Chetouani F, Couve E, Daruvar Ad, Dehoux P, Domann E, Dominguez-Bernal G, Duchaud E, Durant L, Dussurget O, Entian KD, Fsihi H, Portillo FG, Garrido P, Gautier L, Goebel W, Gomez-Lopez N, Hain T, Hauf J, Jackson D, Jones LM, Kaerst U, Kreft J, Kuhn M, Kunst F, Kurapkat G, Madueno E, Maitournam A, Vicente JM, Ng E, Nedjari H, Nordsiek G, Novella S, Pablos Bd, Perez-Diaz JC, Purcell R, Remmel B, Rose M, Schlueter T, Simoes N, Tierrez A, Vazquez-Boland JA, Voss H, Wehland J, Cossart

P: Comparative genomics of Listeria species. buy LDK378 Science 2001, 294:849–852.PubMed 19. Bierne H, Sabet C, Personnic N, Cossart P: Internalins: a complex family of leucine-rich repeat-containing proteins in Listeria monocytogenes . Microbes Infect 2007, 9:1156–1166.PubMedCrossRef 20. Milillo SR, Wiedmann M: Contributions of six lineage-specific internalin-like genes to invasion

efficiency of Listeria monocytogenes . Foodborne Pathog Dis 2008, 6:57–70.CrossRef 21. Roberts A, Nightingale K, Jeffers G, Fortes E, Kongo JM, Wiedmann M: Genetic and phenotypic characterization Bacterial neuraminidase of Listeria monocytogenes lineage III. Microbiology 2006, 152:685–693.PubMedCrossRef 22. Nielsen R: Statistical tests of selective neutrality in the age of genomics. Heredity 2001, 86:641–647.PubMedCrossRef 23. Simonsen K, Churchill G, Aquadro C: Properties of statistical tests of neutrality for DNA polymorphism data. Genetics 1995, 141:413–429.PubMed 24. Bakker HC, Didelot X, Fortes ED, Nightingale KK, Wiedmann M: Lineage specific rates and microevolution in Listeria monocytogenes . BMC Evol Biol 2008, 8:277.CrossRef 25. Wiedmann M, Bruce JL, Keatine C, Johnson AE, McDonough PL, Batt CA: Ribotypes and virulence gene polymorphisms suggest three distinct Listeria monocytogenes lineages with differences in pathogenic potential. Infect Immun 1997, 65:2707–2716.PubMed 26. Orsi RH, Sun Q, Wiedmann M: Genome-wide analyses reveal lineage specifi contributions of positive selection and recombination to the evolution of Listeria monocytogenes. BMC Evol Biol 2008, 8:233.PubMedCrossRef 27. Salcedo C, Arreaza L, Alcala B, Fuente L, Vazquez JA: Development of a multilocus sequence typing method for analysis of Listeria monocytogenes clone. J Clin Microbiol 2003, 41:757–762.PubMedCrossRef 28.