In bold are the locations shared by the four O157:H7 strains The

In bold are the locations shared by the four O157:H7 strains. The direct repeats (duplication are in red). IS629 sites were numbered from 1 – 47 starting with all sites in Sakai, followed by all additional, unshared sites from EDL933, EC4115, the sites found in the plasmids and unshared sites of strain TW1435. The newly found IS629 insertion in O rough:H7 strain MA6 was numbered IS.39. (DOC 200 KB) Additional file 4: “”Table S3″”. IS629 target site presence/absence in CC strains from the O157:H7 stepwise evolutionary model. (XLS 56 KB) Additional file 5: “”Table Doramapimod clinical trial S4″”. Primer sequences for the amplification

of each flanking IS629 regions on the four E. coli genomes available (see Additional Table 2). If IS absent size equal to 0 bp means that the primer pair was designed with one target region inside IS629 therefore the IS629 target site could not be observed. (DOCX 22 KB) References 1. Feng P:

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M, Nakayama K, Terajima J, Watanabe H, Hayashi T: Inference of the impact of insertion sequence (IS) elements on bacterial genome diversification through analysis of small-size structural polymorphisms in Escherichia coli O157 genomes. Genome Res 2009, 19:1809–1816.PubMedCrossRef 8. Arbeit RD: Laboratory procedures for the epidemiologic analysis of microorganisms. In Manual of clinical microbiology. 6th edition. Edited by: Murray PJ, Baron EJ, Pfaller MA, Tenover FC, Yolken RH. Washington, D.C.: ASM Press; 1995:190–208. 9. Whittam TS, Wolfe ML, Wachsmuth IK, selleck kinase inhibitor Orskov F, Orskov I, Wilson RA: Clonal relationships among Escherichia coli strains that cause hemorrhagic colitis and infantile diarrhea. Infect Immun 1993, 61:1619–1629.PubMed 10.

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