At this point of the productive chain, legal control of possible wildlife products is difficult. Common in the commerce of northern Argentina, skins of two wild species, the carpincho and the collared peccary, look very similar to each other ZD1839 research buy and to those of the domestic pig; it is extremely difficult to differentiate them after
they have been tanned. Because there was no an adequate methodology to discriminate between leather of these three species, we developed a new methodology of DNA extraction from skin and leather. This new method involves digesting a leather sample using proteinase K, followed by precipitation of proteins with 5 M NaCl, cleaning with absolute isopropanol and DNA precipitation with 70% ethanol. DNA is hydrated in Tris-EDTA buffer. This protocol provided good-quality DNA suitable for analysis with molecular markers. This new protocol has potential for use in identifying leather products of these species using molecular markers selleck chemicals based on RAPDs.”
“The present study
investigates the magnetic properties of spherical monodispersed maghemite (gamma-Fe2O3) nanoparticles coated with multiple silica (SiO2) layers of different thicknesses, forming core-shell multifunctional nanomaterials. This study was performed using a combination of local probe techniques (Mossbauer spectrometry) and magnetization measurements. At room temperature, both techniques confirm the superparamagnetic state of the samples, even after being coated with the SiO2 shells. The zero-field-cooling-field-cooling magnetization curves of the silica-coated gamma-Fe2O3 nanomaterials with different shell thicknesses allow the evaluation of the intensity of the interparticle click here dipole-dipole interactions. We estimate the interparticle energy within the framework of dipolar interaction models and relate it with the hyperfine
parameters. We further observe that this dipole-dipole interaction increases the superparamagnetic energy barrier, which largely depends on the interparticle distance. Finally, we consider the effect of spin canting at the surface (“”dead layer”") of uncoated gamma-Fe2O3 nanoparticles manifested by a layer of similar to 0.5(1) nm. (C) 2011 American Institute of Physics. [doi: 10.1063/1.3583652]“
“Objective. The objective of this study was to examine the diagnostic accuracy of the different secondary reconstruction slice thicknesses of cone beam computed tomography (CBCT) on artificially created mandibular condyle fractures.
Methods. A total of 63 sheep heads with or without condylar fractures were scanned with a NewTom 3G CBCT scanner. Multiplanar reformatted (MPR) views in 0.2-mm, 1-mm, 2-mm, and 3-mm secondary reconstruction slice thicknesses were evaluated by 7 observers. Inter-and intraobserver agreements were calculated with weighted kappa statistics.