We provide an overview of the benefits and limitations of intrathecal ziconotide treatment and review potential future developments in this new drug class.”
“This study was designed to express the onion fructosyltransferase by Escherichia coli DH5 alpha, and obtain the optimal conditions of FST-1 activity. Thereby, fructosyltransferase gene was obtained by RT-PCR from onion
in this experiment, and named FST-1. The expressed proteins were analyzed by SDS-PAGE. FST-1 activity was identified Romidepsin by the high performance liquid chromatography (HPLC). The optimal conditions of FST-1 were analyzed by the dinonylnaphthalene sulfonic acid (DNS) and orthogonal test. Results revealed https://www.selleckchem.com/products/U0126.html that FST-1 was identified to 98% similarity with fructosyltransferase mRNA of onion (accession number: AJ006066). FST-1 was successfully expressed in E. coli DH5a. HPLC results indicated that the expressed protein from FST-1 had a good transferring activity for fructose. The optimal conditions of FST-1 in catalyzing reaction were the pH 5.0, 45 degrees C and 60% sucrose substrate. The results
in this experiment would lay the foundation for the large-scale of kestose by bio-catalysis method.”
“Production of agro-industrial waste pollutants has become a major problem for many industries. However, agro-industrial wastes also can provide alternative substrates for industry and their utilization in this manner may help solve pollution problems. The aim of this study was to isolate yeasts from wastewater treatment plants that could be used to remove pollutants such as glycerol, paraffin and crude oil from the agro-industrial wastewater. In this study a total of 300 yeast isolates were obtained from samples of agro-industrial wastes, and two strains (M1 and M2) were investigated for their ability to produce valuable products such as lipase and citric
acid. Identification tests showed that these isolates belonged to the species Yarrowia lipolytica. The Y. lipolytica M1 and M2 strains produced maximum levels of lipase (11 and 8.3 U/ml, respectively) for on olive oil, and high levels of citric acid (27 and 8 g/l, respectively) on citric acid fermentation medium.”
“Nattokinase was produced by batch and fed-batch culture of Bacillus subtilis in flask and fermentor. Effect of supplementing complex media (peptone, yeast extract, or tryptone) was investigated on the production of nattokinase. In flask culture, the highest cell growth and nattokinase activity were obtained with 50 g/L of peptone supplementation. In this condition, nattokinase activity was 630 unit/ml at 12 h. In batch culture of B. subtilis in fermentor, the highest nattokinase activity of 3400 unit/ml was obtained at 10 h with 50 g/L of peptone supplementation.