We highlight the convenience of the system for monitoring root development; roots were more sensitive to the chemicals than shoots and leaves. Proline is shown as a potential marker for ecotoxicoxicity. The in vitro system is proposed as a simple and reliable method to test for ecotoxicity in terrestrial plants. (C) 2014 Elsevier Ltd. All rights reserved.”
“The new electrophilic trifluormethylating 1-(trifluoromethyl)-benziodoxole reagents A and B (Scheme I) have been used to selectively attach CF(3) groups to the Satom of cysteine side chains of alpha- and beta-peptides (up to 13-residues-ling: products 7-14).
Other functional groups in the substrates (amino, amido, carbamate, carboxylate, hydroxy, phenyl) are not attacked by these soft reagents. Depending on the conditions, the indole ring of a Trp residue may also be trifluoromethylated
(in the 2-position). The products are purified by chromatography, and identified by (1)H-, (13)C-, and (19)F-NMR spectroscopy, Selleckchem MK 2206 by CD spectroscopy, and by high-resolution mass spectrometry. The CF(3) groups, thus introduced, may be replaced by H (Na/NH(3)), an overall Cys/Ala conversion. The importance of trifluoromethylations in medicinal chemistry and possible applications of the method (spin-labelling imaging, PET) are discussed.”
“Matrix solid-phase dispersion (MSPD) is a simple and cheap sample preparation procedure allowing for the reduction of organic solvent consumption, exclusion of sample component degradation, improvement of extraction efficiency and selectivity, elimination of additional sample clean-up and pre-concentration step before chromatographic click here analysis.\n\nThe paper shows the possibility of MSPD application for qualitative and quantitative analysis of essential oil components in the following herbs: thyme (Thymus vulgaris selleck chemicals llc L.), mint (Mentha piperita), sage (Salvia officinalis L), chamomile (Chamomilla recutita L.), marjoram (Origanum majorana L.), savory (Satureja hortensis L.), and oregano (Origanum vulgare).
The results obtained using MSPD are compared to two other sample preparation methods: steam distillation (SD) and pressurized liquid extraction (PLE).\n\nThe results presented in the paper prove that the total amount and the composition of the essential oil component obtained by MSPD are equivalent to those gained by one of the most effective extraction technique, PLE. (C) 2009 Elsevier B.V. All rights reserved.”
“The Gram-positive bacterial pathogen Streptococcus pyogenes produces a C3 family ADP-ribosyltransferase designated SpyA (S. pyogenes ADP-ribosyltransferase). Our laboratory has identified a number of eukaryotic protein targets for SpyA, prominent among which are the cytoskeletal proteins actin and vimentin. Because vimentin is an unusual target for modification by bacterial ADP-ribosyltransferases, we quantitatively compared the activity of SpyA on vimentin and actin. Vimentin was the preferred substrate for SpyA (k(cat), 58.5 +/- 3.