This makes comparing and contrasting between studies difficult and could potentially lead to erroneous conclusions. The details of these varying factors are discussed in Section 4. Table 2 lists all identified

flavonol compounds detected across all samples, including systematic names and identifying ions. In total eleven flavonol compounds were positively identified. Myricetin was detected in relatively few accessions, but predominantly in Eruca. Previously this flavonol has not been identified in Diplotaxis species (to the authors’ knowledge), however, in this study it was detected in the commercial variety Wild Grazia. Kaempferol glucosides kaempferol-3-glucoside (Astragalin) and kaempferol-3-diglucoside-7-glucoside have only been previously reported in Eruca species, but were additionally detected in two Diplotaxis varieties Wortmannin mw in our study (Wild Grazia and WR2). The ion fragments present in Table 2 confirmed their presence in these two commercial varieties. Kaempferol-3,4′-diglucoside was detected in both genera as reported by Pasini et selleck inhibitor al. (2012) and Martinez-Sanchez, Llorach, Gil,

Ferreres, and Martínez-Sanchez (2007). The only kaempferol glucoside that was exclusive to Eruca species was kaempferol-3-(2-sinapoyl-glucoside)-4′-glucoside. A similar situation was observed for quercetin glucosides. Quercetin-3-glucoside (Isoquercetrin) has only been previously reported in Eruca species, however it was also detected in one commercial accession of Diplotaxis (Wild Grazia). The converse was also found with quercetin-3,3,4′-triglucoside, quercetin-3,4′diglucoside-3′-(6-caffeoyl-glucoside) Chloroambucil and quercetin-3,4′diglucoside-3′-(6-sinapoyl-glucoside), which have only previously

been reported in Diplotaxis. These were detected in several Eruca accessions, as well as in Diplotaxis. Quercetin-3,3,4′-triglucoside showed the correct m/z 787 mass and secondary ions, and quercetin-3,4′diglucoside-3′-(6-caffeoyl-glucoside) was determined by the presence of a characteristic 625 fragment. Quercetin-3,4′-diglucoside-3′-(6-sinapoyl-glucoside) was determined by primary m/z 993 ion and corresponding secondary fragment ions ( Table 2). Two isorhamnetin glucosides were detected in our analysis; isorhamnetin-3-glucoside and isorhamnetin-3,4′-diglucoside. The latter compound was detected in both Eruca and Diplotaxis accessions, as has been reported in other studies ( Martinez-Sanchez, Gil-Izquierdo, Gil, & Ferreres, 2008). Isorhamnetin-3-glucoside has only been previously reported in Eruca, but was also detected in seven Diplotaxis accessions (see Table 4). The concentration of each identified flavonol glucoside is presented in Table 5. As a general, overall observation, it can be said that Diplotaxis accessions have greater concentrations of quercetin flavonol compounds than Eruca, and the converse could be said for kaempferol. However using this as a broad, sweeping view to classify the two genera would be a mistake.