The effect of temperature on the product was studied at different temperatures. The colored product was stable in the temperature range of 0.0�C35��C. At higher temperatures, the drug concentration is increased selleck screening library on prolonged heating due to volatile nature of chloroform. As a result, the absorbance value of the colored products was increased. However, the resultant product was stable for more than 6 h at 25 �� 5��C. The validity of the method for the assay of tablets was determined. The percentage recovery experiments revealed good accuracy of the data. There is no need for the separation of soluble excipients present in marketed tablets as the results were always reproducible equivalent to the labeled contents of the preparations. The recovery results of the proposed method were well agreed with the reported RP-HPLC method for gemifloxacin tablets.

[12] The proposed method has been found to be new, accurate, simple, economic, sensitive, precise, and convenient and is suitable for routine analysis in a laboratory. It can be used in the determination of gemifloxacin in bulk drugs and its pharmaceutical preparations in a routine manner. The results were calculated and reported by utilizing the Smiths Statistical Package (SSP) software. ACKNOWLEDGMENTS The authors are thankful to Sigma Aldrich, Mumbai, India, for providing the pure gemifloxacin sample. The authors also thank the Chairman, Dadhichi College of Pharmacy, Odisha, India, for providing facilities to carry out this work. Footnotes Source of Support: Nil Conflict of Interest: None declared.

Paracetamol (PARA) is chemically N-(4 hydroxyphenyl) acetamide.[1,2] It is used mainly as an analgesic and antipyretic. It is official in the Indian Pharmacopeia[3] [Figure 1]. Nabumetone (NAB) is chemically 4-(6-methoxynaphthalen-2-yl) butan-2-one. It is a nonsteroidal anti-inflammatory drug. It is used in the treatment of rheumatoid arthritis and osteoarthritis. It is official in the United States Pharmacopoeia.[4] Several methods are reported for the individual estimation of PARA and NAB.[5�C7] For PARA, other methods are reported like spectrophotometrically,[8] High Performance GSK-3 Liquid Chromatography with UV detector (HPLC-UV)[9] individually and in combination with other drugs. Literature survey also reveals methods like spectrophotometrically,[10] Liquid Chromatography Mass Spectrometry (LC-MS/MS),[11] and HPLC[12] for estimation of nabumetone individually and in combination with other drugs. No ultraviolet (UV) spectrophotometric method is reported for the simultaneous estimation of PARA and NAB area under curve method (AUC). This paper describes the development and validation of a method to simultaneously quantify PARA and NAB by AUC in bulk and tablet dosage form.