Results: Cytotoxicity of H2O2 exposure was concentration-dependent (30% death with 250 mu M; 87% with 500 mu M at 8 h) and time-dependent (7% at 1 h; 30% at 8 h with 250 mu M H2O2). Catalase abolished such relations. H2O2 increased PLC gamma(1) expression more than that of PLC delta(1) and almost doubled total PLC enzymatic activity between 2 and 8 h, changes prevented by catalase.
The PLC inhibitor U73112 (3 mu M) enhanced the cytotoxic concentration and time effects of H2O2. In acute studies, H2O2 rapidly caused tyrosine phosphorylation of PLC gamma(1). Conclusion: H2O2 increased SC75741 PLC gamma(1) expression and almost doubled total PLC activity, changes abolished by catalase. We conclude that H2O2 is cytotoxic to cultured VSMCs of renal preglomerular Defactinib cost arterioles, a process that is attenuated by compensatory increases in PLC gamma(1) protein level, tyrosine phosphorylation of PLC gamma(1) and PLC enzymatic activity to generate IP3. Copyright (C) 2007 S. Karger AG, Basel.”
“This study deconvolves the temporal dynamics of the neural processes underlying line bisection judgements (i.e., the landmark task).
Event-related potentials (ERPs) were recorded from 96 scalp electrodes in 10 healthy right-handed male subjects while they were judging whether horizontal lines were correctly prebisected. In the control task, subjects judged whether or not the horizontal line was transected by a vertical line, irrespective of its position. Using a current density reconstruction approach, source maxima in the time range from 50 to 400 ms after stimulus onset were
localized and the time courses of activation were elaborated. Five regions, corresponding to those revealed by our previous fMRI studies (e.g., [Fink, G. R., Marshall, J. C., Shah, N. J., Weiss, P. H., Halligan, P. W., Grosse-Ruyken, et al. (2000). Line bisection judgments implicate right parietal cortex and cerebellum as assessed by fMRI. Neurology, 54, 1324-1331]), were identified as contributing significant source activity related to line Aspartate bisection judgements: right middle occipital gyrus (Brodmann area; BA18); bilateral inferior occipital gyrus (BA 19); right superior posterior parietal cortex (BA7) and right inferior posterior parietal cortex (BA40). Temporal deconvolution indicated sequential activation of these regions starting at BA18 as early as 90 ms post-stimulus onset, followed by the successive activation of the right superior posterior parietal (BA7), bilateral inferior occipital (BA19) and right inferior posterior parietal cortex (BA40). Three of these areas (BA18, BA17 and BA19) became reactivated within 250 ms of stimulus onset.
The data provide evidence for an early involvement of the right hemispheric parietal network in visuo-spatial information processing.