Resistance-trained practitioners often consume a high-protein diet along with creatine supplements in an attempt to enhance power/strength and lean mass. The alleged “H 89 price kidney overload” caused by creatine (and its by-product creatinine) and excessive protein ingestion merits further investigation. Therefore, the purpose of this study was to examine the effects of creatine supplementation on kidney function in resistance-trained individuals consuming a high-protein diet. In most of the previous human studies involving creatine supplementation, kidney function was assessed via serum creatinine or its derivative

equations. However, the spontaneous conversion of creatine into creatinine [13] may falsely suggest decreased kidney function in creatine-supplemented Selleckchem BV-6 individuals [8]. To overcome this potential drawback, we used a gold standard method – 51Chromium-ethylenediamine tetraacetic acid (51Cr-EDTA) clearance – to accurately BI 10773 solubility dmso measure glomerular filtration rate in this study. Methods Subjects Young healthy males who regularly engaged in resistance training for at least 1 year and were ingesting a high-protein diet (≥ 1.2 g/Kg/d; which is a usual prescription to resistance-trained practitioners [14]) were eligible to participate. The exclusion criteria included: vegetarian diet, use of creatine supplements in the past 6 months, chronic kidney disease, and use of anabolic steroids.

The participants were advised to maintain their habitual diet. Participants’ characteristics are presented in Table 1. The study was approved by the Ethical Advisory Committee from the School of Physical Education and Sport, University of Sao Paulo. All of the participants signed the informed consent. This trial was registered at clinicaltrials.gov as NCT01817673. Table 1 Participants’ characteristics   Creatine (n = 12) Placebo (n = 14) Age (years) 24 (3) 27 (5) Height (m) 1.79 (0.08) 1.78 (0.05) Weight (Kg) 80.4 (10.3) 78.4 (12.4)

BMI (Kg/m2) 24.8 (1.6) 24.7 Galactosylceramidase (2.9) Training experience (years) 5 (2) 7 (3) Training frequency (sessions per week) 5 (1) 4 (1) Data expressed as mean (standard deviation). Experimental protocol A 12-week, double-blind, randomized, placebo-controlled trial was conducted between July 2011 and February 2013 in Sao Paulo, Brazil. The participants were randomly assigned to receive either creatine or placebo in a double-blind fashion. All of the participants continued with their usual resistance training routines throughout the study. The participants were assessed at baseline (Pre) and after 12 weeks (Post). 51Cr-EDTA clearance was performed to measure the glomerular filtration rate. Additionally, blood samples and twenty-four-hour urine collection were obtained following a 12-h overnight fasting for kidney function assessments. Dietary intake was assessed by 7-day food diaries.