* P < 0.05. Table 1 HER-2/neu mRNA expression Group ΔCt -ΔΔCt 2-ΔΔCt Talazoparib price HER-2 transfected 97.16 ± 0.71

2.62 ± 0.71 6.15 (3.75–10.06)* pcDNA3.1 transfected 9.88 ± 1.10 0.1 ± 0.10 1.07 (1.06–1.08) Non-transfected 9.78 ± 1.09 0 ± 1.09 1 (0.47-2.13) * P < 0.05. Transfected with pcDNA3.1-HER2 in Ishikawa cells induced the increase of COX-2, PGE2 and P450arom expression Western blotting demonstrated that levels of COX-2 and P450arom in Ishikawa cells stably transfected with pcDNA3.1-HER2 were significantly higher compared to those in empty plasmid-transfected or non-transfected cells (Figure 2). In additionally, ELISA analysis showed that the supernatant level of PEG2 in pcDNA3.1-HER2-transfected group was significant higher than that of the empty plasmid-transfected group, and the normal cell group. Transfected with pcDNA3.1-HER2 induced the increase of autocrine E2 from Ishikawa cells ELISA indicated was there were statistically significant differences in the cell supernatants of E2 levels among

the pcDNA3.1-HER2-transfected group, the empty plasmid-transfected group, and the normal VS-4718 mouse cell group (Table 2). Table 2 ELISA analyses for PGE 2 and E 2 in the supernatants of endometrial carcinoma cells Group PGE2(pg/ml) E2 (pg/ml) Transfected 41.69 ± 0.87* 31.49 ± 2.14* pcDNA3.1 transfected 31.35 ± 1.06 21.16 ± 2.37 Non-transfected 27.67 ± 1.20 20.56 ± 3.27 * P < 0.05. Inhibition of HER2 in Ishikawa cells induced the decrease of COX-2 and P450arom expression RNA interference technology was used for the down-regulation of HER2 expression in Ishikawa cells. As shown in Figure 3, HER2 siRNAs were effectively able to knockdown the levels of HER2 in Ishikawa cells. Interestingly, down-regulation of HER2 expression induced significantly the reduction of COX-2 and P450arom levels in Ishikawa cells (Figure 3). Figure 3 The levels of COX-2, and P450armo in the

ishikawa cells this website transfencted with HER2 siRNA. A. Represent image for western blot. B. Analysis of protein levels in each group and quantification of band density was done using Image J. * P < 0.05. Inhibition of COX-2 in the over-expressed HER2 Ishikawa cells led to the decrease of PGE2 and P450arom expression To further investigate the relationship between the Phosphoglycerate kinase COX-2/PGE2/P450arom signal and HER2, celecoxib, a selective COX-2 inhibitor, was used for inhibition experiment. The results showed that inhibition of COX-2 in the over-expressed HER2 Ishikawa cells led to the obvious decrease of PGE2 and P450arom expression (Figure 4; Table 3). Figure 4 The levels of P450armo in the ishikawa cells treated with 80 μM celecoxib. A. Represent image for western blot. B. Analysis of protein levels in each group and quantification of band density was done using Image J. * P < 0.05. Table 3 ELISA analysis for PGE 2 in the supernatants of tranfected endometrial carcinoma cells treated with Celecoxib Group Celecoxib – (pg/ml) Celecoxib + (pg/ml) pcDNA3.