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“In this work, the volatile compounds of cinnamon barks from three species and seven habitats were detected and identified for the first time by GC-MS and FTIR analysis. GC-MS analysis indicated that the main compound in the volatile oils of nine samples was trans-cinnamaldehyde Dibutyryl-cAMP concentration (66.28-81.97%). Cinnamomum loureirii had the highest volatile oil yield (3.08%) and in that yield was a high percentage of trans-cinnamaldehyde (81.97%).
Cinnamomum verum contained significant quantities of eugenol, and Cinnamomum cassia had more abundant alpha-guaiene compared with C. loureirii and C. verum. Hierarchical cluster analysis, similarity evaluation and principal component analysis showed that the nine samples were effectively identified and evaluated. Load factor analysis revealed that the differences in the volatile compounds of the nine samples were mainly reflected in the aldehyde, alcohol, alkane and eugenol contents. Our research indicated that GC-MS and FTIR techniques, combined with chemometrics, proved to be an effective strategy for identifying the species and for determining their geographical distribution, especially in the assessment of cinnamon quality for use in raw herbal medicines. (C) 2012 Elsevier B.V. All rights reserved.”
“As part of an epidemiological study on legionellosis, we attempted to isolate Legionella spp. from hot spring www.selleckchem.com/products/GSK1904529A.html water and were able to isolate L. londiniensis
HYKF-90505 (=JCM 16338), confirming that L. londiniensis inhabits
hot spring water in Japan. To investigate the disease potential of L. londiniensis, Selleck BMS-777607 we examined its ability to grow intracellularly within Acanthamoeba sp. JAC/E1 strain. The isolated HYKF-90505 was able to grow within Acanthamoeba sp. JAC/E1 strain, and we confirmed also that the HYKF-90505 strain showed cytotoxicity for cultured cells such as J774.1 (JCRB0018). However, in a culture of human U937 cells, the bacterial count was not increased by the intracellular growth of the HYKF-90505 strain. Cells infected for 24 h and stained using the Gim,nez method showed no intracellular growth of the HYKF-90505 strain. Thus, the isolate appears to be weakly pathogenic to humans.”
“Purpose: To develop a proniosomal carrier system of curcumin for transdermal delivery.
Methods: Proniosomes of curcumin were prepared by encapsulation of the drug in a mixture of Span 80, cholesterol and diethyl ether by ether injection method, and then investigated as a transdermal drug delivery system (TDDS). The formulated systems were characterized for size, drug entrapment, angle of repose, hydration rate and vesicular stability under various storage conditions. In vitro release studies were performed using albino rat skin.
Results: The method used for preparing proniosome resulted in an encapsulation yield of 82.3 – 86.8%. Scanning electron microscopy analysis showed that the surface of the particles was smooth.