In the present study, the peptide consisting of N-terminal residues 1–20 of EV71 VP4 of genotype C4 was fused to hepatitis B core antigen (HBcAg) and expressed in E. coli. The check details resulting fusion proteins were able to spontaneously assemble into chimeric VLPs, which elicited virus-neutralizing antibody response. We further identified a highly conserved linear neutralizing epitope in the N-terminus of EV71 VP4 by epitope mapping experiments.

Our results suggest that chimeric HBcAg particles carrying a neutralizing epitope of EV71 VP4 could be a promising vaccine candidate against EV71 infection. selleck products Results Generation of chimeric particles carrying the peptide VP4N20 The gene sequence and amino acid sequence of peptide VP4N20 as well as its insertion position in HBcAg are shown in Figure 1. The plasmid vector pET22b (+) (Novagen) encodes a six-histidine tag at the C-terminal region of recombinant proteins for convenient purification by affinity chromatography as well as expression analysis by Western-blot. A carboxyl-terminally truncated HBcAg protein (149 aa, HBc-N149) and a fusion protein (HBc-N149-VP4N20) were expressed in E. coli, respectively. Figure 1 Schematic presentation of the chimeric

HBcAg protein construct. The shaded box represents the N-terminal 20 a.a. of VP4 of Bj08 and BrCr-TR. Italics letters indicate nucleotide sequences, and the percentages indicate the degree of conservation among the 100 strains of EV71 from Asia. The efficient expression of both Stattic mouse proteins was demonstrated by Western-blot after IPTG induction (Figure 2A). They were further purified using Ni Sepharose column. The purity of proteins was evaluated by densitometric analysis after staining with Coomassie blue and the representative samples of expressed Interleukin-3 receptor proteins were shown in Figure 2B. Since HBcAg protein can form particles both in vivo and in vitro, we then investigated whether the recombinant proteins can form particles. Electron microscopy analysis showed that both HBc-N149 and

HBc-N149-VP4N20 proteins were able to efficiently form particles with the size around 25–30 nm (Figure 3). The results suggest that the chimeric proteins can self-assemble to form VLPs. Figure 2 Protein expression and purification. The expression of HBc-N149 and HBc-N149-VP4N20 protein was detected by Western blot. (A) Lane 1: HBc-N149-VP4N20. Lane 2: HBc-N149. Lane 3: Negative control. The protein purification was visualized by SDS-PAGE. (B) Lane 1: Uninduced bacteria expressing HBc-N149-VP4N20; Lane 2: Induced bacteria expressing HBc-N149-VP4N20; Lane 3: Purified HBc-N149-VP4N20. Figure 3 Electron microphotographs of HBc-N149 and HBc-N149-VP4N20 particles. (A) Particles assembled from HBc-N149. (B) Chimeric particles assembled from HBc-N149-VP4N20. Size bar: 50 nm.