however, in a few cases, stronger expression was observed in the apical region of the epithelium. Lam inin gamma 2 was not observed in the stromal cells. There was no significant variation in immunoreactivity between the different menstrual phases. Semi quantitative evaluation of laminin gamma 2 To compare laminin not gamma 2 expression in the ectopic and eutopic endometrium, we evaluated the percentage of positive glands in each tissue. No differ ences were noted between eutopic and ectopic endome tria from patients with endometriosis. however, in patients without endo metriosis, the endometrium displayed the lowest Inhibitors,Modulators,Libraries percent age of laminin gamma 2 chain expressing glands. To better compare the intensity of lam inin gamma 2 chain expression in different tissues, a semi quantitative scoring method was performed using whole tissue sections as described above.
When we evaluated only the intensity of laminin gamma 2 staining in positive glands from the different Inhibitors,Modulators,Libraries tissues, no differences between the groups were observed. however, eutopic endometrium from women without endometriosis exhibited a null score more frequently than eutopic and ectopic endometrium from patients with endometriosis. In other words, when glands were positive in EuE. their intensity was lower than that of lesions, at a higher magnification, laminin gamma 2 also appeared in the cytoplasm of epithelial cells, either distributed along the basement membrane or with a more uneven distribution as illustrated in Figure 3A C.
In areas of desquamation, the epithelial cells of the dis persing glands displayed a highly intensified intracyto plasmic immunoreactivity, whereas laminin gamma 2 Inhibitors,Modulators,Libraries was detected in a more linear pattern along the basement membranes of intact glands. In pluristratified glands, laminin gamma 2 chain immu noreactivity was more irregular. positive glands in EuE. When a global scoring method was applied that considered both the number of positive glands and their intensity, no differences were observed between ectopic and eutopic endometrium in patients with endometriosis. However, endometrium from patients without endometriosis displayed a reduced global expres sion score compared either with eutopic endometrium from patients with endometriosis. either with ectopic endometrium. Discussion In this study, LAMC2 mRNA was found to be differen tially expressed in the ectopic endometrium of women with endometriosis compared with their eutopic endomet Inhibitors,Modulators,Libraries rium.
The role of the laminin gamma 2 chain in the pathogenesis of endometriosis has not been previ ously evaluated, although some studies have implicated this protein in cancer invasion and Inhibitors,Modulators,Libraries metastasis. Therefore, we hypothesised that laminin gamma 2 chain selleck chem inhibitor could also play a role in the adhesion, migration and invasion of endometrial cells, which are required for the development of endometriosis.