..H to V FG…H to V – - A to C – G44 [A to D] – [A to D] – - – - – - [A to D A to D [A to D] G46 (ST25) LCL161 [A to E] CDE CDE – - – - – - CDE [ ] [ ] G47 (abn, aby) [A to R] BL – L – BL – - – [B to R] [B to R] [B to R] G51 (abc) [A to G] – [A to G] [A to G] – [A to G] – - B to L [A to G] C [A to G] G57 (acb) [A to H] M to AG – - – [ ] – - -
[ ] [ ] – ORFs in each island are referrred to by capital letters. Brackets denote ORFs flanking genomic islands. Conserved genomic regions are highlighted in bold. Dots between letters denote that corresponding ORFs are not contiguous. #Genomic regions larger than those identified
in A. baumannii. A high number of GEIs is conserved in the genome of the Acinetobacter sp. Defactinib chemical structure strain DR1. Interestingly, dot plot analyses showed that gene order is more similar between A. baumannii AB0057 strain and Acinetobacter sp. strain DR1 than between the same A. baumannii strain and A. baylyi (Figure 5). According to rpoB sequence analysis, DR-1 strain belongs to the A. calcoaceticus-A. baumannii complex, and is closely related (99.7% identity) to gen. sp. “”Between 1 and 3″” [3]. Figure 5 Dot plot comparisons of Acinetobacter genomes. The degree of relatedness of the A. baylyi and Acinetobacter sp. DR1 chromosomes to the A. baumannii AB0057 chromosome is buy JQEZ5 illustrated by dot plot comparisons. Genomic regions in A. baumannii strains of different genotypes The distribution of 18 genomic islands in the A. baumannii Mannose-binding protein-associated serine protease population was monitored by PCR analyses. Coding DNA regions of 600-1500 bp, representative
of each GEI, were amplified from the DNA of 23 A. baumannii strains associated with 21 epidemics that occurred in 14 hospitals of the Mediterranean area from 1999 to 2009, including the sequenced 3909 and 4190 strains used as control. Nearly all the strains were representative of cross-transmission episodes, and were isolated with identical PFGE types from more than two patients of the same or different institutions [9]. Strains belong to eight different STs, and 10/23 strains are ST2. PCR data are summarized in Table 4. Taking into account that negative data may denote partial island deletion or polymorphism in sequences targeted by the primers, the conservation of islands seems to vary significantly among the analyzed strains. G43 and G51 had been found in most strains but not in the two strains assigned to ST78 and some strains assigned to ST2.