Figure 2 shows the phylogenetic neighborhood of Bradyrhizobium sp. strain WSM1417 in a 16S rRNA sequence based tree. This strain clusters closest to Bradyrhizobium canariense LMG 22265T and Bradyrhizobium japonicum LMG 6138T with 99.85% selleck chemicals llc and 99.48% sequence identity, respectively. Figure 1 Images of Bradyrhizobium sp strain WSM1417 using scanning (Left) and transmission (Center) electron microscopy as well as light microscopy to visualize colony morphology on a solid medium (Right). Table 1 Classification and general features of Bradyrhizobium sp. strain WSM1417 according to the MIGS recommendations [11,12]. Figure 2 Phylogenetic tree showing the relationships of Bradyrhizobium sp. strain WSM1417 (shown in blue print) with some of the root nodule bacteria in the order Rhizobiales based on aligned sequences of the 16S rRNA gene (1,334 bp internal region).

All sites … Symbiotaxonomy Bradyrhizobium sp. WSM1417 is poorly effective on L. angustifolius, producing only 45% of the dry matter compared to that achieved by the commercial inoculant strain Bradyrhizobium sp. WSM471 on this species. In contrast on L. mutabilis, WSM1417 performs much better, yielding 83% of the dry matter produced by WSM471 on this same host. Genome sequencing and annotation information Genome project history This organism was selected for sequencing on the basis of its environmental and agricultural relevance to issues in global carbon cycling, alternative energy production, and biogeochemical importance, and is part of the Community Sequencing Program at the U.S.

Department of Energy, Joint Genome Institute (JGI) for projects of relevance to agency missions. The genome project is deposited in the Genomes OnLine Database [22] and an improved-high-quality-draft genome sequence in IMG. Sequencing, finishing and annotation were performed by the JGI. A summary of the project information is shown in Table 2. Table 2 Genome sequencing project information for Bradyrhizobium sp. strain WSM1417. Growth conditions and DNA isolation Bradyrhizobium sp. strain WSM1417 was grown to mid logarithmic phase in TY rich medium [23] on a gyratory shaker at 28��C. DNA was isolated from 60 mL of cells using a CTAB (Cetyltrimethylammonium bromide) bacterial genomic DNA isolation method [24]. Genome sequencing and assembly The genome of Bradyrhizobium sp.

strain WSM1417 was sequenced at the Joint Genome Institute (JGI) using a combination of Illumina [25] and 454 technologies [26]. An Illumina GAii shotgun library which generated 82,690,654 reads totaling 6,284.5 Mb, and a paired end 454 library with an average insert size of 10 kb which generated 770,255 reads totaling 144.4 Mb of 454 data were generated for this genome. All general aspects of library construction and sequencing performed at the JGI Brefeldin_A can be found at the JGI website [24]. The initial draft assembly contained 2 contigs in 1 scaffold. The 454 paired end data was assembled with Newbler, version 2.3.