Fig. S3. A genomic comparison between three strains of Photorhabdus, showing syntenic regions within the tca pathogenicity island. Fig. S4. A genomic comparison between four strains of Photorhabdus, showing syntenic regions within the tcb pathogenicity island. Fig. S5. A genomic comparison between TSA HDAC four strains of Photorhabdus,
showing syntenic regions within the tcd pathogenicity island. Fig. S6. A genomic comparison between three strains of Photorhabdus, showing syntenic regions across the PirAB toxin locus. Fig. S7. A genomic comparison between two strains of Photorhabdus asymbiotica, showing syntenic regions across the PVC cif region. Fig. S8. A genomic comparison between three strains of Photorhabdus, showing syntenic regions across a Type III secretion locus TTSS-1. Fig.
S9. A genomic comparison between three strains of Photorhabdus, showing syntenic regions across a Type III secretion locus which is identical in the P. asymbiotica strains and absent from the P. luminescens TTO1 strain. Table S1. Summary statistics for the different assemblies resulting from the three different workflows, termed A, B and C. Appendix S1. Supplementary methods. Please note: Wiley-Blackwell is not responsible for the content or functionality of ICG-001 any supporting materials supplied by the authors. Any queries (other than missing material) should be directed to the corresponding author for the article. “
“The Flavobacterium psychrophilum gliding motility N (GldN) protein was investigated to determine its ability to elicit antibody responses and provide protective immunity in rainbow trout Oncorhynchus mykiss (Walbaum). GldN was PCR-amplified, cloned into pET102/D-TOPO, and expressed in Escherichia coli. Bacteria expressing recombinant GldN (rGldN) were formalin-inactivated and injected intraperitoneally
(i.p.) into rainbow trout with Freund’s complete adjuvant (FCA) in four separate studies that used two different immunization protocols followed by challenge evaluations. Fish injected with E. coli only in FCA served as the control. Antibody responses to F. psychrophilum new whole-cell lysates measured by ELISA were low in all four studies. Protection against F. psychrophilum challenge was observed in the first study, but not in the three following studies. The discrepancies in results obtained in the later studies are unclear but may relate to formalin treatment of the antigen preparations. Overall, it appeared that rGldN delivered i.p. as a crude formalin-killed preparation is not a consistent vaccine candidate, and more work is required. Additionally, this study illustrates the importance of conducting multiple in vivo evaluations on potential vaccine(s) before any conclusions are drawn. “
“l-isoleucine-4-hydroxylase (IDO) is a recently discovered member of the Pfam family PF10014 (the former DUF 2257 family) of uncharacterized conserved bacterial proteins.