DESIGN: Sputum from gold miners
was cultured on MGIT and U. We estimated cost per culture, and, for smear-negative samples, incremental cost per additional M. tuberculosis gained with MGIT using a decision-tree model.
RESULTS: Among 1267 specimens, MGIT vs. LJ gave a higher yield of mycobacteria (29.7% vs. 22.8%), higher LY2606368 clinical trial contamination (16.7% vs. 9.3%) and shorter time to positive culture (median 14 vs. 25 days for smear-negative specimens). Among smear-negative samples that were culture-positive on MGIT but negative/contaminated on LJ, 77.3% were non-tuberculous mycobacteria (NTM). Cost per culture on LJ, MGIT and MGIT+LJ was respectively US$12.35, US$16.62 and US$19.29. The incremental cost per additional M. tuberculosis identified by standard biochemical tests and microscopic cording was respectively US$504.08 and US$328.10 using MGIT vs.
LJ, or US$160.80 and US$109.07 using MGIT+LJ vs. U alone.
CONCLUSION: MGIT gives higher yield and faster results at relatively high cost. The high proportion of NTM underscores the need for rapid speciation tests. Minimising contaminated cultures is key to cost-effectiveness.”
“Aim: In experimental studies, lysyl oxidase like-1 (LOX-L1) (-/-) mice were shown to have similar pelvic floor dysfunction to female rats. LOX-L1 levels in endopelvic fascia decrease as a result of increasing births in women with pelvic prolapse. For these reasons, we investigated the LOX-L1 gene polymorphism, which check details has an important
role in connective tissue and collagenous metabolism in stress urinary incontinence (SUI). Materials and Methods: A total of 87 women with SUI who underwent normal vaginal delivery and 87 controls were involved in the study. Single nucleotide gene polymorphisms in LOX-L1′s rs1048661, G > T, pArg141Leu, Exon-1 SmaI; rs3825942, C > T, pGly153Asp, Hinf-1 and rs2165241, C > T, Intron-1 BsrI regions OSI-906 cell line were searched. The results were statistically compared as alleles with 3 x 2 ?2-test. Results: A total of 32 (34%) GG, 20 (21%) GT, 42 (45%) TT, 32 (37%) GG, 43 (39%) GT, 21 (24%) TT polymorphisms in rs1048661; 30 (36%) CC, 16 (19%) CT, 37 (45%) TT, 41 (59%) CC, 15 (22%) CT, 13 (19%) TT polymorphisms in rs2165241; and 63 (72%) CC, 21 (24%) CT, 3 (4%) TT; 48 (6%) CC, 22 (30%) CT, 3 (4%) TT polymorphisms in rs3825942 were found in patients and the control group, respectively. In patients, the TT polymorphism in the rs1048661 and rs2165241 region were found to be significant. Conclusions: The homozygote TT polymorphism in the rs1048661 and rs2165241 region of LOX-L1 gene may be responsible from SUI physiopathology.”
“it an attractive method for routine laboratory assays.SETTING: Polymerase chain reaction (PCR) offers great promise for the rapid, sensitive and specific diagnosis of tuberculous meningitis (TBM).