43. Bordier C: Phase separation of integral membrane proteins in Triton-X114 solution. J Biol Chem 1981, 256:1604–1607.PubMed

44. Duffy MF, Noormohammadi AH, Baseggio N, Browning GF, Markham PF: Immunological and biochemical characterization of membrane proteins. In Methods in Molecular Biology. Edited by: Miles R, Nicholas R. Humana Press, Totowa, New Jersey; 1998:279–298. vol. 104 45. Ladefoged SA, Christiansen G: Mycoplasma hominis expresses two variants of a cell-surface protein, one a lipoprotein, and one not. Microbiology 1998,144(Pt 3):761–770.PubMedCrossRef 46. Inukai M, Takeuchi M, Shimizu K, Arai M: Mechanism of action of globomycin. J Antibiot (Tokyo) 1978,31(11):1203–1205.CrossRef 47. Inukai M, Ghrayeb J, Nakamura K, Inouye M: Apolipoprotein, an intermediate in JPH203 supplier the processing selleck products of the major lipoprotein of the Escherichia coli outer membrane. J Biol Chem 1984,259(2):757–760.PubMed 48. O’Brien-Simpson NM, Pathirana RD, Paolini RA, Chen YY, Veith PD, Tam V, Ally N, Pike RN, Reynolds EC: An immune response directed to proteinase and adhesin functional epitopes protects against Porphyromonas gingivalis-induced periodontal bone loss. J Immunol 2005,175(6):3980–3989.PubMed Competing interests The authors declare that they have no competing interests. Authors’ contributions I.S.P designed the study, performed the experiments and

data analysis, and drafted the manuscript, AK helped with the experiments, CC Salubrinal price contributed the ltuf siglac construct, P.D.V and M.D.G performed mass C-X-C chemokine receptor type 7 (CXCR-7) spectrometry identification and analysis and provided suggestions about the manuscript. G.F.B and P.F.M contributed to the study design, analysis, drafting and review of the manuscript. All authors have read and approved the manuscript.”
“Background Enterococci are normal constituents of the gastro-intestinal flora of humans and other animals [1–3]. Although they only occasionally cause infections in healthy individuals,

they are the third most commonly isolated gram positive organisms from hospital-associated (HA) infections in the United States and are increasingly reported in other countries [4, 5]. Enterococcal infections are often difficult to treat due to the number of antibiotics to which these organisms are resistant. Some antibiotic resistances are intrinsic, such as resistances to cephalosporins, while other antibiotic resistances are acquired through mutations or horizontal gene transfer, most notably the van systems that encode vancomycin resistance [6–12]. Several recent studies also confirmed that enterococci can transfer their resistance to even more virulent organisms, such as Staphylococcus aureus[13]. Enterococcus faecalis is the most common enterococcal species recovered from infections. However, in the last decade, infections with Enterococcus faecium have been on the rise in the United States, Europe, and South America [2–5, 14]. In the US, isolates of E. faecium now account for ca.