18) 51(51.52) 1.130 0.288   female 11(11.11) 19(19.19)     Age(year) ≤ 60 9(9.09) 30(30.30) 1.200 0.273   > 60 20(20.20) 40(40.40)     Tumor diameter(cm) ≤ 5 17(17.17) 40(40.40) 4.175 0.041   > 5 12(12.12) 10(10.10)     Histological grade

1 5(5.05) 16(16.16) 2.030 0.566   2 13(13.13) 27(27.27)       3 11(11.11) 27(27.27)     Invasion depth T 1 0(0.00) 11(11.11) 6.116 0.106   T 2 6(6.06) 17(17.17)       T 3 10(10.10) 21(21.21)       T 4 13(13.13) 21(21.21)     Lymph node metastasis N 0 3(3.03) 27(27.27) 10.227 0.017   N 1 15(15.15) 20(20.20)       N 2 7(7.07) 19(19.19)       N 3 4(4.04) 4(4.04)   #BEZ235 clinical trial randurls[1|1|,|CHEM1|]#   TNM stage II 2(2.02) 19(19.19) 8.108 0.044   III 4(4.04) 10(10.10)       IV 13(13.13) 31(31.31)       IV 10(10.10) 10(10.10)     Lymphatic vessel infiltration positive 28(28.28) 27(27.27) 27.636 0.000   negative 1(1.01) 43(43.43)     Vascular infiltration positive 28(28.28) 15(15.15) 46.624 0.000   negative 1(1.01) 55(55.56)     Table 2 Logistic analysis on the correlation of CD 133 protein expression with clinicopathological parameters (n = 99 cases) Parameter this website B SE Wald df Sig. Exp(B) 95.0%CI for Exp(B) Gender 0.012 0.017 0.201 1 0.328 1.003

0.972~7.873 Age(year) 0.007 0.018 0.158 1 0.691 1.007 0.875~3.125 Tumor diameter(cm) 0.209 0.123 2.908 1 0.088 1.233 1.334~8.911 Invasion depth -1.238 0.488 6.430 1 0.011 0.290 1.079~12.381 Histological grade 0.181 0.281 0.414 1 0.520 1.198 0.987~3.212 Lymph node metastasis -0.929 0.459 4.102 1 0.043 0.395 1.156~18.324 TNM stage 1.048 0.636 2.720 1 0.049 2.853 1.138~14.216 Lymphatic vessel infiltration 0.847 0.601 1.568 1 0.067 3.213 1.335~10.954 Vascular infiltration 0.760 0.662 1.317 1 0.251 2.137 0.991~6.872 CD133 mRNA expressions in primary lesion and in NCGT The semi quantitative RT-PCR detection in 31 patients was performed to confirm the expressions of CD133 mRNA in primary lesion (100.0%) and NCGT (16.1%, 5 cases/31 cases)(χ2 = 15.125, P = Thiamet G 0.000) (Figure 2A). Figure 2 Detection and distribution of semi-quantitative BSV of CD133 mRNA by RT-PCR (n = 31 cases). Note: 2A showed the detection of semi-quantitative BSV of CD133 mRNA. A and C showed CD133 mRNA expressions in primary lesions. E and G showed CD133 mRNA expressions in NCGT. B and D showed GAPDH mRNA expressions as an internal reference for subgroup of primary lesions.