This antibody detected ~43 KDa protein bands. Expression of UBE2Q2 Protein in Colorectal
Tissues Tissues from the cancerous part of 43 colorectal tumors along with their normal counterparts were subjected to western blot analysis for the UBE2Q2 protein. The individual characteristics of the colorectal cancers are summarized in table 1. Increased levels of UBE2Q2 immunoreactivity were observed in the Inhibitors,research,lifescience,medical cancerous tissues related to 28 (65.11%) of the specimens when compared with their corresponding normal tissues (figure 3). No significant changes were observed in the cancerous cells of 10 (23.26%) of the specimens as compared with their corresponding normal tissues (figure 3). Five (11.63%) of the samples showed lower expression levels of UBE2Q2 in their affected cells. UBE2Q2 bands in western blots from 43 cancerous and their corresponding Inhibitors,research,lifescience,medical normal tissues revealed intensities of respectively 4957±591 and 3305±451 (mean±SEM) arbitrary units. These differences were statistically significant at P<0.001 (paired t test). Table 1 Clinicopathologic information of the 43 patients with colorectal
cancer Figure 3 Expression of UBE2Q2 in colorectial Inhibitors,research,lifescience,medical tumors and their corresponding normal tissues. A) Immunoblot analysis using UBE2Q2 antibody (detecting ~43 KDa protein bands) against the extracts from four normal human colorectal tissue samples (N) and their corresponding … Discussion Carcinogenesis in CRC is a multistep event Inhibitors,research,lifescience,medical that includes a progressive accumulation of genetic alterations in multiple genes whose protein products are regulated by the UPS.3,4 Here, we report the expression pattern of a novel E2-conjugating enzyme, UBE2Q2, in colorectal carcinoma cell lines and colorectal PF299 price primary tumors. Our results showed that all the colorectal carcinoma cell lines tested express UBE2Q2 protein and also its transcript. Under our experimental conditions, the line Caco2 showed the highest levels of both UBE2Q2 mRNA and protein, whereas SW742, LS180, and SW1116 showed
Inhibitors,research,lifescience,medical relatively lower levels of expression. This difference may be due to the variation in the characteristics of these cell lines. There are properties in each cell line which are used to classify second CRC cell lines such as morphological markers, gland formation, and modal chromosome number.22,23 Thus, SW1116, which expresses low levels of UBE2Q2, falls into Group III of the classification made by Drewinko et al.,22 while a high UBE2Q2-expressing cell, like SW48, corresponds to Group I of the same classification. The high and low UBE2Q2-expressing cells also differ in other features such as amounts of production of growth factors and carcinoembrionic antigen (CEA).24 The production of CEA and some growth factors such as TGF-α, TGF-β, and platelet-derived growth factor (PDGF)-like material is significantly higher in SW742 cell line.